Wang Ziju, Wei Xinhong, Piao Lizhen, Zhang Xiaofei, Wang Hong
Department of Pediatrics, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.
Department of Ultrasound, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.
Front Cell Infect Microbiol. 2025 May 22;15:1600470. doi: 10.3389/fcimb.2025.1600470. eCollection 2025.
Norovirus (NoV) is a leading cause of acute gastroenteritis in pediatric populations worldwide. However, the role of gut microbiota in NoV pathogenesis remains poorly understood.
We conducted a longitudinal metagenomic analysis of fecal samples from 12 NoV-infected children and 13 age-matched healthy controls in Northeast China. Microbial composition and functional pathways were assessed using high-throughput shotgun sequencing and bioinformatic profiling.
NoV infection was associated with significant gut microbial dysbiosis, including increased alpha diversity and distinct taxonomic shifts. Notably, , spp., and were enriched in infected individuals. Functional analysis revealed upregulation of metabolic pathways involved in carbohydrate and lipid processing. These microbial and functional alterations persisted over time and correlated with disease severity.
Our findings reveal novel associations between NoV infection and gut microbiota dysbiosis, particularly the enrichment of , which may influence host-pathogen interactions via metabolic or immune mechanisms. The identified microbial and metabolic signatures offer potential biomarkers for diagnosis and targets for microbiota-based therapeutic strategies in pediatric NoV infection.
诺如病毒（NoV）是全球儿童急性胃肠炎的主要病因。然而，肠道微生物群在诺如病毒发病机制中的作用仍知之甚少。
我们对中国东北地区12名诺如病毒感染儿童和13名年龄匹配的健康对照的粪便样本进行了纵向宏基因组分析。使用高通量鸟枪法测序和生物信息学分析评估微生物组成和功能途径。
诺如病毒感染与显著的肠道微生物失调有关，包括α多样性增加和明显的分类学变化。值得注意的是，[此处原文缺失具体菌属名称]菌属在感染个体中富集。功能分析显示参与碳水化合物和脂质加工的代谢途径上调。这些微生物和功能改变随时间持续存在，并与疾病严重程度相关。
我们的研究结果揭示了诺如病毒感染与肠道微生物群失调之间的新关联，特别是[此处原文缺失具体菌属名称]菌属的富集，这可能通过代谢或免疫机制影响宿主-病原体相互作用。所确定的微生物和代谢特征为儿科诺如病毒感染的诊断提供了潜在的生物标志物，并为基于微生物群的治疗策略提供了靶点。
