Manapkanova Ulzhan, Rymkhanova Nazgul, Reim Stefanie, Fritzsche Eric, Höfer Monika, Beshko Natalya, Satekov Yeskendir, Kushnarenko Svetlana V
Institute of Plant Biology and Biotechnology, 45 Timiryazev Str., Almaty 050040, Kazakhstan.
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, 71 Al-Farabi Av., Almaty 050040, Kazakhstan.
Plants (Basel). 2025 May 30;14(11):1676. doi: 10.3390/plants14111676.
This study investigates the phenotypic characteristics, genetic diversity, and population structure of four wild cherry species collected from various regions of Kazakhstan and Uzbekistan: Pall., (Nevski) Gilli, var. (Pojark.) Ingram, and (Franch.). A total of 163 accessions were characterized morphologically using standardized descriptors for plant, leaf, and fruit traits. Genetic diversity was assessed using 13 simple sequence repeat (SSR) markers. STRUCTURE analysis revealed that 87.7% of the accessions were assigned to pure species. However, hybrid accessions were identified in var. (34.4%), (18.5%), and (8.0%). Identical genotypes were found across all species, with showing the highest proportion (54.8%), likely due to clonal propagation via root suckers. Among the four species, exhibited the highest genetic diversity, while had the lowest. Analysis of molecular variance (AMOVA) showed that genetic variation within the species (81%) was substantially greater than variation among the species (19%). These findings enhance our understanding of the genetic relationships among wild cherry species in Central Asia and provide valuable data for conservation planning and breeding programs aimed at improving drought and frost tolerance in species.
本研究调查了从哈萨克斯坦和乌兹别克斯坦不同地区收集的四种野生樱桃物种的表型特征、遗传多样性和种群结构： Pall.、 (Nevski) Gilli、 var. (Pojark.) Ingram和 (Franch.)。使用针对植物、叶片和果实性状的标准化描述符对总共163份种质进行了形态学特征分析。使用13个简单序列重复（SSR）标记评估遗传多样性。STRUCTURE分析表明，87.7%的种质被归类为纯物种。然而，在 var. （34.4%）、 （18.5%）和 （8.0%）中发现了杂交种质。在所有物种中都发现了相同的基因型，其中 的比例最高（54.8%），这可能是由于通过根蘖进行克隆繁殖。在这四个物种中， 的遗传多样性最高，而 的遗传多样性最低。分子方差分析（AMOVA）表明，物种内的遗传变异（81%）远大于物种间的变异（19%）。这些发现增进了我们对中亚野生樱桃物种间遗传关系的理解，并为旨在提高 物种耐旱性和抗冻性的保护规划和育种计划提供了有价值的数据。
