Poulsen L K, Weeke B
Allergy. 1985 Aug;40(6):405-16. doi: 10.1111/j.1398-9995.1985.tb02679.x.
A method is described in which the conventional radioallergosorbent test (RAST) for determination of allergen-specific IgE in serum has been modified by displacing the solidphase sorbent by a gel of aluminium hydroxide (Al(OH)3). The optimum conditions for this new analysis (AlRAST) are determined, taking into account speed, costs and the ratio between responses of patients and normal individuals. The AlRAST fulfills the criteria for validity: it is specific and shows parallelism between serum dilution curves. The sensitivity and reproducibility are slightly lower than in the conventional RAST, but sufficient for most routine and screening procedures. The preparation of allergosorbents in AlRAST can be accomplished within 1 h, thus making it convenient for fast screening of patients against unusual allergen extracts, as well as for allergen standardization from dose-response curves. Further, it is possible to standardize aluminium hydroxide adsorbed extracts used for immunotherapy by using the products directly as allergosorbents in the AlRAST.
本文描述了一种方法，其中用于测定血清中过敏原特异性IgE的传统放射变应原吸附试验（RAST）已通过用氢氧化铝（Al(OH)₃）凝胶替代固相吸附剂进行了改进。考虑到速度、成本以及患者与正常个体反应之间的比率，确定了这种新分析方法（AlRAST）的最佳条件。AlRAST符合有效性标准：它具有特异性，并且血清稀释曲线之间显示出平行性。其灵敏度和重现性略低于传统RAST，但对于大多数常规和筛查程序来说已足够。AlRAST中变应原吸附剂的制备可在1小时内完成，因此便于针对不常见的过敏原提取物快速筛查患者，以及根据剂量反应曲线进行过敏原标准化。此外，通过直接将用于免疫治疗的氢氧化铝吸附提取物作为AlRAST中的变应原吸附剂使用，可以对其进行标准化。
