Wu Tong, Zhou Quan, Zheng Chunfu, Zou Xiaodong
Kobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong Kong, Shenzhen, Guangdong Province, China.
Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol. 2025;2940:141-150. doi: 10.1007/978-1-0716-4615-1_13.
Complex interactions between viruses and host cells are difficult to study via traditional models. Organoids, three-dimensional (3D) tissue-like structures derived from stem cells, have emerged as transformative tools for modeling human tissues and revealing the intricate dynamics of virus-host interactions. This chapter summarizes the relevant applications of organoids in virus-host interaction studies and provides detailed information and experimental procedures for using them to study virus-host interactions.
病毒与宿主细胞之间的复杂相互作用很难通过传统模型进行研究。类器官是由干细胞衍生而来的三维（3D）组织样结构，已成为用于模拟人体组织和揭示病毒-宿主相互作用复杂动态的变革性工具。本章总结了类器官在病毒-宿主相互作用研究中的相关应用，并提供了使用它们研究病毒-宿主相互作用的详细信息和实验步骤。
