Shoaib Muhammad, Ali Muhammad Muddassir, Tang Minjia, Weiwei Wang, Zhang Xuejing, He Zhuolin, Wu Zhongyong, Wang Shengyi, Hao Baocheng, Li Ruichao, Pu Wanxia
Key Laboratory of New Animal Drug Project, Gansu Province/Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs/Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
One Health. 2025 Jun 6;20:101101. doi: 10.1016/j.onehlt.2025.101101. eCollection 2025 Jun.
Extended-spectrum beta-lactamase (ESBL)-producing resists broad-spectrum cephalosporins, considered as a critical priority pathogen, and its presence in animals, humans, and the environment highlights its significance as a One Health issue. Dairy farm waste is a potential environmental contaminant and can serves as a significant reservoir for the emergence and spread of ESBL-producing , which belongs to One Health clones and poses a serious global threat. This study aimed to investigate the occurrence and genomic characteristics of ESBL-producing clones of One Health concern from dairy farm waste in Gansu, China. In this study, we isolated and characterized two CTX-M-55 ESBL-producing strains, ZYX8158 and ZYS8091, which belong to One Health clones. The genomic analysis revealed a large resistome, mobilome, virulome, and plasmidome was acquired by both ESBL-producing strains. The genome-based typing revealed that ZYX8158 and ZYS8091 belonged to globally disseminated clones ST10 (O73:H31 serotype) and ST2325 (O66:H25 serotype), respectively. Phylogeographical analysis revealed both strains as potential One Health clones due to their clustering with related strains isolated from animal, human, and environmental sources, regardless of geographical boundaries, indicating their zoonotic potential and clonal spread in the One Health sector. This study highlights that dairy farm waste can be a potential source of the emergence and dissemination of One Health clones of critical priority ESBL-producing in One Health settings, which demands continuous and integrated genomic surveillance for comprehensive knowledge and mitigation strategies.
产超广谱β-内酰胺酶（ESBL）的细菌对广谱头孢菌素耐药，被视为关键优先病原体，其在动物、人类和环境中的存在凸显了其作为“同一健康”问题的重要性。奶牛场废弃物是一种潜在的环境污染物，可作为产ESBL细菌出现和传播的重要储存库，这些细菌属于“同一健康”克隆群，对全球构成严重威胁。本研究旨在调查中国甘肃奶牛场废弃物中具有“同一健康”意义的产ESBL克隆群的发生情况和基因组特征。在本研究中，我们分离并鉴定了两株产CTX-M-55 ESBL的菌株ZYX8158和ZYS8091，它们属于“同一健康”克隆群。基因组分析显示，这两株产ESBL的菌株都获得了大量的耐药基因组、可移动基因组、毒力基因组和质粒基因组。基于基因组的分型显示，ZYX8158和ZYS8091分别属于全球传播的克隆群ST10（O73:H31血清型）和ST2325（O66:H25血清型）。系统地理学分析表明，这两株菌株均为潜在的“同一健康”克隆群，因为它们与从动物、人类和环境来源分离的相关菌株聚类，不受地理边界限制，表明它们具有人畜共患病潜力和在“同一健康”领域的克隆传播。本研究强调，奶牛场废弃物可能是“同一健康”环境中关键优先产ESBL“同一健康”克隆群出现和传播的潜在来源，这需要持续和综合的基因组监测，以获取全面的知识和缓解策略。
