Vaudaux P E, Zulian G, Huggler E, Waldvogel F A
Infect Immun. 1985 Nov;50(2):472-7. doi: 10.1128/iai.50.2.472-477.1985.
The mechanisms responsible for the development of a pyogenic infection (most commonly due to staphylococci) in the vicinity of an implanted foreign body have been studied recently by several investigators. Thus, we have been able to demonstrate that the phagocytic function of residential polymorphonuclear leukocytes (PMN) is deficient in the presence of a foreign body. Others have shown that in the presence of foreign surfaces, microorganisms produce extracellular amorphous material, the pathogenic role of which is still to be defined. In the present study we use a novel assay system to demonstrate that Staphylococcus aureus Wood 46, after attachment to polymethylmethacrylate (PMMA), shows increased resistance to the phagocytic-bactericidal action of normal PMN. The first step of this assay involves the reproducible attachment of [3H]thymidine-labeled bacteria to PMMA cover slips. During the second step, attached bacteria were exposed to guinea pig peritoneal exudate PMN. In the third and final step, attached S. aureus cells were removed from the cover slips using a procedure harmless to the bacteria. The extent of bacterial detachment was estimated by radioactive counts and their viability by standard colony counts. Whereas bacteria that were attached artificially and rapidly by centrifugation and immediately exposed to PMN were killed in the phagocytic assay, bacteria adhering spontaneously to the cover slips for a prolonged period of time were more resistant to the killing action of the phagocytes. The spontaneous adherence of S. aureus to PMMA renders it poorly susceptible to the killing action of PMN.
最近，几位研究人员对植入异物附近发生化脓性感染（最常见的原因是葡萄球菌）的机制进行了研究。因此，我们已经能够证明，在异物存在的情况下，常驻多形核白细胞（PMN）的吞噬功能存在缺陷。其他人已经表明，在异物表面存在的情况下，微生物会产生细胞外无定形物质，其致病作用仍有待确定。在本研究中，我们使用一种新型检测系统来证明金黄色葡萄球菌Wood 46在附着于聚甲基丙烯酸甲酯（PMMA）后，对正常PMN的吞噬杀菌作用表现出增强的抵抗力。该检测的第一步涉及将[3H]胸腺嘧啶标记的细菌可重复地附着于PMMA盖玻片上。在第二步中，将附着的细菌暴露于豚鼠腹腔渗出液PMN。在第三步也是最后一步中，使用对细菌无害的程序从盖玻片上除去附着的金黄色葡萄球菌细胞。通过放射性计数估计细菌脱离的程度，并通过标准菌落计数估计其活力。通过离心人工快速附着并立即暴露于PMN的细菌在吞噬试验中被杀死，而长时间自发附着于盖玻片的细菌对吞噬细胞的杀伤作用更具抵抗力。金黄色葡萄球菌对PMMA的自发附着使其对PMN的杀伤作用敏感性较差。
