Shatri Albertina Mariina Ndinelao, Bere Silas Kudakwashe, Bouman Denise, Mumbengegwi Davis Ropafadzo
Department of Human Biological & Translational Medical Sciences, School of Medicine, Hage Geingob Campus, University of Namibia, Windhoek, Namibia.
Department of Oral Surgery, School of Dentistry, Hage Geingob Campus, University of Namibia, Windhoek, Namibia.
J Trop Med. 2025 Jun 16;2025:7544856. doi: 10.1155/jotm/7544856. eCollection 2025.
Medicinal plants are used in Namibia for oral hygiene and to treat oral diseases. Validating the content and efficacy of medicinal chewsticks used in communities helps to provide proof of concept of medicinal plants used as a complementary/alternative medicine for oral diseases. This study presents the first report on quantified phytoconstituents, antimicrobial, time-kill kinetics, and biofilm inhibition properties of organic and aqueous extracts against . Dry plant materials were ground into powder and macerated in methanol and distilled water. Different phytoconstituents were quantified by Folin-Ciocalteu colorimetric method, ferric reducing antioxidant power assay, and DPPH free radical scavenging. An antibacterial assay was performed using the agar well diffusion method and a resazurin 96-well-based assay. Kill-time assay was done at various concentrations over 4 h. Biofilm inhibition was done using the crystal violet method. Higher total flavonoid, total phenol contents, and free radical scavenging abilities were reported in methanol twig extracts. Inhibition zones of 28 ± 0.82 mm, with MICs of 15.6 ± 0.00 μg/mL, are reported against . The bactericidal endpoint of organic extracts for was reached after 4 h of incubation at 8 × MIC (124.8 μg/mL). These were comparable to the positive control, gentamicin. The organic extracts showed ≥ 50% biofilm inhibition against root canal-infecting at concentrations between 7.8 and 500 μg/mL, indicating strong biofilm inhibition. The study demonstrated that crude extracts have promising antibacterial properties and can eradicate biofilms in root canal treatments.
纳米比亚将药用植物用于口腔卫生和治疗口腔疾病。验证社区使用的药用咀嚼棒的成分和功效，有助于为药用植物作为口腔疾病补充/替代药物提供概念验证。本研究首次报告了有机提取物和水提取物针对[具体对象未明确]的定量植物成分、抗菌性能、时间杀菌动力学和生物膜抑制特性。将干燥的植物材料研磨成粉末，并用甲醇和蒸馏水浸渍。采用福林-西奥尔特法、铁还原抗氧化能力测定法和DPPH自由基清除法对不同的植物成分进行定量。使用琼脂孔扩散法和基于刃天青的96孔测定法进行抗菌试验。在4小时内对不同浓度进行杀菌时间测定。使用结晶紫法进行生物膜抑制试验。甲醇树枝提取物中总黄酮、总酚含量和自由基清除能力较高。报告称对[具体对象未明确]的抑菌圈为28±0.82毫米，最低抑菌浓度为15.6±0.00微克/毫升。有机提取物在8×最低抑菌浓度（124.8微克/毫升）下孵育4小时后达到对[具体对象未明确]的杀菌终点。这些结果与阳性对照庆大霉素相当。有机提取物在7.8至500微克/毫升的浓度下对根管感染的[具体对象未明确]表现出≥50%的生物膜抑制作用，表明具有较强的生物膜抑制能力。该研究表明，[具体植物未明确]粗提物具有良好的抗菌性能，并且可以在根管治疗中根除[具体对象未明确]生物膜。
