Scheffel U, Tsan M F, McIntyre P A
J Nucl Med. 1979 Jun;20(6):524-31.
We have evaluated the factors influencing the labeling of human platelets in the presence of autologous plasma. The labeling efficiency was found to be dependent on a) the time and temperature of incubation, b) the platelet concentration, c) the concentration of citrate ions (in ACD anticoagulant), and d) the concentration of 8-hydroxyquinoline in the suspending medium. Contrary to what was expected, unsaturated transferrin was found not to interfere with the transfer of In-111 from the [111In] 8-hydroxyquinoline complex to the platelets. Based on the findings of this study, a protocol was established by which human platelets can be labeled with In-111 in plasma with a labeling efficiency of 55.5 +/- 9.3 (mean +/- 1 s.d.) percent.
我们评估了在存在自体血浆的情况下影响人血小板标记的因素。发现标记效率取决于：a）孵育时间和温度；b）血小板浓度；c）柠檬酸盐离子（在ACD抗凝剂中）的浓度；d）悬浮介质中8-羟基喹啉的浓度。与预期相反，发现不饱和转铁蛋白不会干扰铟-111从[111In] 8-羟基喹啉复合物向血小板的转移。基于本研究的结果，制定了一种方案，通过该方案人血小板可以在血浆中用铟-111进行标记，标记效率为55.5±9.3（平均值±1标准差）%。
