Wang Jianzhong, Zhao Yi, Qiu Jicheng, Liu Jing, Zhou Rui, Ma Xialin, Wu Xiaojie, Li Xiaoguang, Mao Wei, Liu Yiduo, Zhang Heng
Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030810, China.
Laboratory of Veterinary Clinical Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 29, Saihan District, Hohhot 010011, China.
Vet Sci. 2025 May 22;12(6):509. doi: 10.3390/vetsci12060509.
is a globally significant zoonotic pathogen responsible for severe parasitic diseases in humans and animals. This study aimed to design, develop, and evaluate a novel immunochromatographic test (ICT) using a recombinant MIC2-MIC3 fusion protein (rMIC2-MIC3) for detecting specific antibodies against . The ICT demonstrated exceptional sensitivity, capable of detecting -specific antibodies in sera diluted up to 1:8. Specificity evaluation confirmed no cross-reactivity with antibodies against other parasites, such as , and . Stability tests revealed the test strips maintained full functionality after 12 weeks of storage at 24 °C. The coincidence rate of the colloidal gold test strips prepared in this study with a commercial ELISA kit was 94.59%. Comparisons with advanced serodiagnostic tools, such as chimeric antigen-based ELISAs and recombinant protein diagnostics, further highlighted its robustness and applicability. These findings underscore the potential of the rMIC2-MIC3-based ICT as a reliable, economical, and accessible diagnostic tool for toxoplasmosis in veterinary and human medicine.
是一种在全球范围内具有重要意义的人畜共患病原体，可导致人类和动物患上严重的寄生虫病。本研究旨在设计、开发和评估一种新型免疫层析试验（ICT），该试验使用重组MIC2-MIC3融合蛋白（rMIC2-MIC3）来检测针对的特异性抗体。该免疫层析试验表现出卓越的灵敏度，能够检测稀释至1:8的血清中的特异性抗体。特异性评估证实，该试验与针对其他寄生虫（如、和）的抗体无交叉反应。稳定性测试表明，试纸条在24℃储存12周后仍保持全部功能。本研究制备的胶体金试纸条与商用ELISA试剂盒的符合率为94.59%。与基于嵌合抗原的ELISA和重组蛋白诊断等先进血清学诊断工具的比较，进一步突出了其稳健性和适用性。这些发现强调了基于rMIC2-MIC3的免疫层析试验作为兽医和人类医学中弓形虫病可靠、经济且易用的诊断工具的潜力。
