Lim Zi Xiang, Peos Jackson, Ostojic Stefan, Arthur Peter G, Fournier Paul A
Exercise Physiology and Biomarkers Laboratory, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117456, Singapore.
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117456, Singapore.
Antioxidants (Basel). 2025 May 30;14(6):667. doi: 10.3390/antiox14060667.
Oxidative stress contributes to the activation of muscle protein synthesis after high-intensity resistance exercise (HIRE) or low-intensity resistance exercise combined with blood flow restriction (LIBFR), but it is unclear if this oxidative stress response post-exercise is monophasic or multiphasic. We aimed to answer this question using albumin Cys34 oxidation as an oxidative stress marker.
Seven untrained individuals completed HIRE and LIBFR on separate days. Albumin Cys34 oxidation (total and reversibly and irreversibly oxidized fractions), muscle oxygenation, oxygen consumption (V˙O2), lactate, and heart rate (HR) were measured before and up to 5 h post-exercise.
Both HIRE and LIBFR induced a biphasic increase in total oxidized albumin Cys34, with a transient peak in irreversibly oxidized albumin Cys34 immediately post-exercise ( < 0.001) before a delayed sustained increase in reversibly oxidized albumin Cys34, which peaked at 90-120 min and lasted ≥5 h post-exercise ( < 0.05). Muscle oxygenation decreased immediately post-exercise ( < 0.001) before rising above baseline ( < 0.05). V˙O2, HR, and blood lactate peaked post-exercise ( < 0.001) and returned to baseline within 15-90 min. Irreversibly oxidized albumin Cys34 was positively correlated with lactate and V˙O2 post-exercise ( < 0.001).
Here, we show that resistance exercise, with or without blood flow restriction, results in an early biphasic oxidative stress response after exercise.
氧化应激有助于高强度抗阻运动（HIRE）或低强度抗阻运动联合血流限制（LIBFR）后肌肉蛋白质合成的激活，但运动后这种氧化应激反应是单相还是多相尚不清楚。我们旨在使用白蛋白Cys34氧化作为氧化应激标志物来回答这个问题。
7名未经训练的个体在不同日期完成了HIRE和LIBFR。在运动前及运动后长达5小时测量白蛋白Cys34氧化（总氧化、可逆和不可逆氧化部分）、肌肉氧合、耗氧量（V˙O2）、乳酸和心率（HR）。
HIRE和LIBFR均诱导总氧化白蛋白Cys34双相增加，运动后立即出现不可逆氧化白蛋白Cys34的短暂峰值（<0.001），随后可逆氧化白蛋白Cys34出现延迟持续增加，在运动后90 - 120分钟达到峰值并持续≥5小时（<0.05）。运动后立即肌肉氧合下降（<0.001），然后升至基线以上（<0.05）。V˙O2、HR和血乳酸在运动后达到峰值（<0.001），并在15 - 90分钟内恢复到基线。不可逆氧化白蛋白Cys34与运动后乳酸和V˙O2呈正相关（<0.001）。
在此，我们表明，无论有无血流限制，抗阻运动都会导致运动后早期双相氧化应激反应。
