巴格达市鱼类处理人员中[具体疾病或情况未明确]的调查与基因确认
Investigation and genetic confirmation of the in fish handlers in Baghdad city.
作者信息
Obead Noor Majed, Alhaboubi Amer Rasool
机构信息
Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
出版信息
J Adv Vet Anim Res. 2025 Mar 22;12(1):1-7. doi: 10.5455/javar.2025.l866. eCollection 2025 Mar.
OBJECTIVE
The present study aims to investigate molecular confirmation for species in fish handlers in Baghdad City, central Iraq.
MATERIALS AND METHODS
Sixty stool samples were collected between early November 2023 and late April 2024. All samples were examined phenotypically using a modified Ziehl-Neelsen stain and genotypically (nested polymerase chain reaction technique) based on a partial sequence of 18S rRNA genes with sequencing and phylogenetic tree analysis.
RESULTS
The total molecular results identified with an infection rate of 45% (27/60). A higher infection rate of 51.9% (14/27) was found in the age group between 15 and 35 years, and male handlers recorded a lower infection rate (45%) than females (41.6%). April had a higher elevation in the infection rate of 60% (6/10) than other months.
CONCLUSION
The . was the only species found in fish handlers, and these local isolates have higher similarity with other isolates of China and Iran.
目的
本研究旨在对伊拉克中部巴格达市鱼类加工人员中的物种进行分子鉴定。
材料与方法
于2023年11月初至2024年4月底收集了60份粪便样本。所有样本均采用改良齐-尼氏染色法进行表型检查，并基于18S rRNA基因的部分序列，通过测序和系统发育树分析，采用巢式聚合酶链反应技术进行基因分型检查。
结果
分子检测结果显示总感染率为45%（27/60）。15至35岁年龄组的感染率较高，为51.9%（14/27），男性加工人员的感染率（45%）低于女性（41.6%）。4月份的感染率升高至60%（6/10），高于其他月份。
结论
是在鱼类加工人员中发现的唯一物种，这些本地分离株与中国和伊朗的其他分离株具有更高的相似性。
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