Pérez-Martínez Pamela I, Gutiérrez-Espinosa Viridiana, Ávalos-Gómez Christian, De la Garza-Amaya Mireya, Vargas-Ruíz Alejandro, Higuera-Piedrahita Rosa I, Marín-Flamand Ernesto, Lonngi-Sosa Cristal D, González-Díaz Francisco R, Ramírez-Álvarez Hugo, González-Ruíz Cynthia
Molecular Pathology Veterinary Laboratory, Multidisciplinary Research Unit, Facultad de Estudios Superiores Cuautitlán, UNAM, Cuautitlán Izcalli CP 54714, State of Mexico, Mexico.
Virology, Genetics and Molecular Biology Laboratory, Facultad de Estudios Superiores Cuautitlán, UNAM, Cuautitlán Izcalli CP 54714, State of Mexico, Mexico.
Microorganisms. 2025 Jun 10;13(6):1341. doi: 10.3390/microorganisms13061341.
Lactic acid bacteria are components of the gastrointestinal tract microbiota in both humans and animals and are widely used as probiotics. is the most closely related genus to probiotic activity. It is capable of releasing membrane microvesicles (MVs), whose primary functions include carrying and transmitting antigens to host tissues and modulating host defense responses. In the present study, MVs were isolated from resident in the ileum of free-living rats, and their immunostimulant effect was evaluated in two biological models. MVs were characterized using SDS-PAGE electrophoresis, electron microscopy, and nanoparticle tracking analysis. In the first model, the immunostimulatory effect of MVs was evaluated on ovine abomasal explants, which had been previously stimulated with MVs and then challenged with third-stage larvae of . This resulted in a decrease in the percentage of larval association and favored the migration of inflammatory cells to the infection site. In the second model, the macrophage cell line RAW 264.7 was stimulated with MVs to evaluate the expression of transcripts encoding IL-1β and TNF-α. MVs isolated from demonstrate immunostimulatory and probiotic effects in the two biological models assessed. This suggested that the MVs possess similar immunostimulatory effects as those reported for the parent bacteria.
乳酸菌是人和动物胃肠道微生物群的组成部分，被广泛用作益生菌。 是与益生菌活性关系最密切的属。它能够释放膜微泡（MVs），其主要功能包括将抗原携带并传递到宿主组织以及调节宿主防御反应。在本研究中，从自由生活大鼠回肠中的 分离出MVs，并在两种生物学模型中评估其免疫刺激作用。使用SDS-PAGE电泳、电子显微镜和纳米颗粒跟踪分析对MVs进行表征。在第一个模型中，评估了MVs对绵羊皱胃外植体的免疫刺激作用，该外植体先前已用MVs刺激，然后用 的第三期幼虫攻击。这导致幼虫附着百分比降低，并有利于炎症细胞向感染部位迁移。在第二个模型中，用MVs刺激巨噬细胞系RAW 264.7，以评估编码IL-1β和TNF-α的转录本的表达。从 分离出的MVs在评估的两种生物学模型中均表现出免疫刺激和益生菌作用。这表明MVs具有与亲本细菌报道的类似的免疫刺激作用。
