Kang Haiseong, Lee Jonghoon, Han Ji Min, Kim Yong Hoon, Joo Insun, Kim Hyochin
Food Microbiology Division, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation, Cheongju 28159, Republic of Korea.
Microorganisms. 2025 Jun 17;13(6):1415. doi: 10.3390/microorganisms13061415.
Given the limited genetic characterization of methicillin-resistant (MRSA) in South Korea, we performed whole-genome sequencing (WGS) on eight MRSA strains isolated from raw fish products, including 327 sliced raw flatfish, 111 flatfish stew products, 85 sliced raw rockfish, and 11 rockfish stew products. Phylogenetic analysis revealed two distinct clusters-comprising five ST72-t324-SCCIVc strains and two novel sequence types-as well as a singleton strain (ST630-t4549-SCCV). A total of five antimicrobial resistance genes (ARGs), four plasmid replicon genes, three mobile genetic element genes, and seventy-three virulence factor genes were identified, with distinct genetic profiles observed between the clusters. Notably, several MRSA strains were isolated from samples obtained at the same retail market, indicating potential local clustering. Four ST72-t324-SCCIVc strains, collected from the same market, shared identical profiles in terms of four ARGs, two plasmid replicons, two mobile genetic elements, and several virulence factor genes. These findings provide valuable insights into the genomic characteristics of MRSA in raw fish products and highlight the need for ongoing surveillance and monitoring efforts in South Korea.
鉴于韩国耐甲氧西林金黄色葡萄球菌（MRSA）的基因特征有限，我们对从生鱼产品中分离出的8株MRSA菌株进行了全基因组测序，这些生鱼产品包括327片生比目鱼、111份比目鱼炖菜产品、85片生石斑鱼和11份石斑鱼炖菜产品。系统发育分析揭示了两个不同的簇，包括5株ST72-t324-SCCIVc菌株和两种新的序列类型，以及一个单独的菌株（ST630-t4549-SCCV）。共鉴定出5个抗菌抗性基因（ARGs）、4个质粒复制子基因、3个移动遗传元件基因和73个毒力因子基因，在各簇之间观察到不同的基因谱。值得注意的是，从同一零售市场采集的样本中分离出了几株MRSA菌株，表明可能存在局部聚集。从同一市场收集的4株ST72-t324-SCCIVc菌株在4个ARGs、2个质粒复制子、2个移动遗传元件和几个毒力因子基因方面具有相同的谱型。这些发现为了解生鱼产品中MRSA的基因组特征提供了有价值的见解，并突出了韩国持续监测和监督工作的必要性。
