Thirumalai Anbazhagan, Sharmiladevi Palani, Girigoswami Koyeli, Prabhu Alex Daniel, Girigoswami Agnishwar
Medical Bionanotechnology, Faculty of Allied Health Sciences (FAHS), Chettinad Hospital & Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai, TN-603103, India.
Medical Bionanotechnology Lab, Department of Obstetrics and Gynaecology, Centre for Global Health Research, Saveetha Medical College, Saveetha Institute of Medical and Technical Sciences, Thandalam, Chennai, 602105, India.
ADMET DMPK. 2025 Jun 18;13(3):2790. doi: 10.5599/admet.2790. eCollection 2025.
Multifunctional hybrid nanoparticles garner heightened interest for prospective biomedical applications, including medical imaging and medication administration, owing to their synergistic benefits of constituent components. Therefore, we demonstrated an optimized protocol for synthesizing magnetofluorescent nanohybrids comprising fluorescent carbon dots with magnetic nanoparticles.
Carbon dot-coated iron oxide nanoparticles (CDs@FeO) were synthesized with varying citric acid concentrations by a one-pot hydrothermal synthesis method for the development of a low-cost and biocompatible contrast agent (CA) for enhanced multimodal imaging (fluorescent and and weighted magnetic resonance imaging (MRI)) to replace the conventional CAs.
The physicochemical characterization of the synthesized CDs@FeO revealed that 3 g of citric acid used for the synthesis of nanoparticles, keeping Fe(II) and Fe(III) ratio 1:2 provides higher stability of -78 mV zeta potential, saturation magnetization of 24 emu/g, with a hydrodynamic diameter of 68 nm. Carbon coating affects surface spins on FeO, resulting in fewer surface-based relaxation centres, making relaxation relatively more prominent. Furthermore, the surface-engineered iron oxide NPs are efficient in producing both and weighted MRI as well as fluorescence-based imaging. The molar relaxivity and molar radiant efficiency derived from phantom studies demonstrate their effectiveness in multimodal medical imaging. The cytotoxicity assay, haemolysis assay, haematology, and histopathology studies show that the optimized CDs@FeO are biocompatible, haemocompatible, and negligibly toxic.
We can conclude the significant potency of CDs@FeO for multimodal diagnosis.
多功能杂化纳米粒子因其组成成分的协同优势，在包括医学成像和药物给药在内的潜在生物医学应用中引起了越来越多的关注。因此，我们展示了一种优化的方案，用于合成包含荧光碳点和磁性纳米粒子的磁荧光纳米杂化物。
通过一锅水热合成法，用不同浓度的柠檬酸合成了碳点包覆的氧化铁纳米粒子（CDs@FeO），以开发一种低成本且生物相容的造影剂（CA），用于增强多模态成像（荧光成像和加权磁共振成像（MRI）），以取代传统的CA。
合成的CDs@FeO的物理化学表征表明，用于合成纳米粒子的3 g柠檬酸，保持Fe(II)和Fe(III)比例为1:2，可提供更高的稳定性，ζ电位为-78 mV，饱和磁化强度为24 emu/g，流体动力学直径为68 nm。碳涂层影响FeO上的表面自旋，导致基于表面的弛豫中心减少，使得弛豫相对更显著。此外，表面工程化的氧化铁纳米粒子在产生加权和加权MRI以及基于荧光的成像方面都很有效。体模研究得出的摩尔弛豫率和摩尔辐射效率证明了它们在多模态医学成像中的有效性。细胞毒性试验、溶血试验、血液学和组织病理学研究表明，优化后的CDs@FeO具有生物相容性、血液相容性，且毒性可忽略不计。
我们可以得出结论，CDs@FeO在多模态诊断方面具有显著潜力。
