Toya Ryohei, Okuno Miki, Sasaki Yosuke, Yoshihara Keisuke, Deguchi Yuichiro, Nagano Debora Satie, Shimada Seiji, Ogura Yoshitoshi
Production Veterinary Medical Center, Miyazaki Prefecture Agricultural Mutual Aid Association, Miyazaki, Japan.
Department of Infectious Medicine, Kurume University School of Medicine, Fukuoka, Japan.
NPJ Antimicrob Resist. 2025 Jun 30;3(1):59. doi: 10.1038/s44259-025-00130-2.
Although extensively studied, the association between antimicrobial usage and the emergence of antimicrobial resistance (AMR) in livestock still has unresolved aspects. This study analyzed the genomes of 195 Escherichia coli strains from pigs, a species with high antimicrobial consumption, across five production stages on 13 farms in Japan employing diverse antimicrobial administration strategies. A total of 61 acquired AMR genes (aARGs), spanning 13 distinct antimicrobial classes, were identified. A significant correlation was found between antimicrobial usage and the number of aARGs in E. coli strains. The four farms with the highest usage administered antimicrobials orally as routine prophylaxis during fattening. These farms showed significantly higher proportions of multidrug-resistant (MDR) genotypes at all stages compared to farms without routine prophylaxis. The number of frequently detected aARGs was more strongly correlated with total antimicrobial usage than with the usage of the corresponding antimicrobial classes. Co-occurrence network analysis suggested that genetic linkages among these aARGs may promote co-selection, thereby acting as a driving force in the emergence of MDR strains under routine prophylaxis treatment.
尽管已进行了广泛研究，但牲畜抗菌药物使用与抗菌药物耐药性（AMR）出现之间的关联仍存在未解决的方面。本研究分析了来自猪的195株大肠杆菌的基因组，猪是抗菌药物消费量很高的物种，这些菌株来自日本13个农场的五个生产阶段，采用了不同的抗菌药物给药策略。共鉴定出61个获得性AMR基因（aARG），涵盖13个不同的抗菌药物类别。研究发现抗菌药物使用与大肠杆菌菌株中aARG的数量之间存在显著相关性。使用量最高的四个农场在育肥期间将抗菌药物作为常规预防措施口服给药。与没有常规预防措施的农场相比，这些农场在所有阶段的多重耐药（MDR）基因型比例显著更高。经常检测到的aARG数量与抗菌药物总使用量的相关性比与相应抗菌药物类别的使用量更强。共现网络分析表明，这些aARG之间的遗传联系可能促进共选择，从而在常规预防治疗下成为MDR菌株出现的驱动力。
