Sultan Omer Sheriff, Jonathan Goh Chong Ming, Lin Seow Liang, Davamani Fabian, Sidhu Preena, Choudhury Minati
A.T. Still University-MOSDOH-Missouri School of Dentistry and Oral Health, Kirksville, USA.
School of Dentistry, IMU University, Kuala Lumpur, Malaysia.
Saudi Dent J. 2025 Jul 1;37(4-6):24. doi: 10.1007/s44445-025-00019-w.
This study evaluates the antimicrobial efficacy of gold nanogel as an endodontic medicament against Enterococcus faecalis in persistent root canal infections. It investigates whether gold nanogel can serve as an effective and biocompatible alternative to conventional medicaments in eliminating E. faecalis. Gold nanoparticles (< 100 nm) were tested against E. faecalis using the agar diffusion method. Extracted teeth inoculated with E. faecalis were treated with gold nanogel (1000 µg/mL), chlorhexidine gluconate (2%), calcium hydroxide, or saline for seven days. Antimicrobial efficacy was assessed on days 1, 3, and 7 using agar diffusion and colony-forming unit (CFU) assays. Gold nanogel significantly reduced E. faecalis biofilm viability, showing greater efficacy than calcium hydroxide and comparable results to 2% chlorhexidine. Chlorhexidine eradicated E. faecalis by day 7, while gold nanogel exhibited sustained antibacterial action with minimal CFUs remaining. Gold nanogel demonstrated superior efficacy over calcium hydroxide and comparable effectiveness to chlorhexidine gluconate (2%) against E. faecalis. Further studies are recommended to evaluate its clinical applications and long-term biocompatibility.
本研究评估了金纳米凝胶作为一种根管治疗药物对持续性根管感染中粪肠球菌的抗菌效果。研究其是否能作为一种有效且生物相容性良好的替代传统药物来消除粪肠球菌。使用琼脂扩散法对小于100纳米的金纳米颗粒进行了针对粪肠球菌的测试。对接种了粪肠球菌的离体牙用金纳米凝胶（1000微克/毫升）、葡萄糖酸氯己定（2%）、氢氧化钙或生理盐水处理7天。在第1、3和7天使用琼脂扩散法和菌落形成单位（CFU）测定法评估抗菌效果。金纳米凝胶显著降低了粪肠球菌生物膜的活力，显示出比氢氧化钙更高的疗效，且与2%葡萄糖酸氯己定的结果相当。到第7天时葡萄糖酸氯己定根除了粪肠球菌，而金纳米凝胶表现出持续的抗菌作用，剩余的CFU极少。金纳米凝胶对粪肠球菌显示出优于氢氧化钙的疗效，且与2%葡萄糖酸氯己定的效果相当。建议进一步研究以评估其临床应用和长期生物相容性。
