Gadratagi Basana Gowda, Mandal Lopamudra, Sah Rameswar Prasad, C Anilkumar, G Guru-Pirasanna-Pandi, Patil Naveenkumar B, Sahu Nandini, P Muhammed Azharudheen T, Behera Sasmita, Rath Prakash Chandra, Das Mohapatra Shyamaranjan
Crop Protection Division, ICAR-Central Rice Research Institute, Cuttack, Odisha, 753006, India.
ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra, 413255, India.
J Appl Genet. 2025 Jul 2. doi: 10.1007/s13353-025-00985-1.
The Asian rice gall midge poses a severe threat to rice yields, making the development of resistant rice cultivars the most cost-effective and efficient strategy to manage the gall midge. A diverse panel of 115 rice accessions was phenotyped, revealing varying resistance to the gall midge biotype 2. The panel's diversity and familial relatedness were assessed before conducting a genome-wide association study to identify marker-trait associations (MTAs) for gall midge resistance. Newly developed candidate gene-derived markers were used along with random microsatellite markers in genotyping. A total of 50 significant MTAs with P < 0.05 were found. Except for chromosome 11, all of the rice chromosomes had significant MTAs. The QTL identified on chromosomes 6, 8, and 9 has been associated with 66F 67R, 54F 55R, and RM107, explaining maximum phenotypic variation. The allele effects of the associated markers differentiated susceptible and highly resistant genotypes, confirming their association with gall midge resistance. Seven genes associated with the general response to stress tolerance were found in the gall midge resistance QTL region. On chromosome 9, one putative gene for gall midge resistance was identified, which is associated with marker RM23914. These candidate genes identified have a significant impact on the gall midge resistance response. This investigation contributes to a better understanding of the rice gall midge resistance mechanism and provides essential genetic information for the breeding and functional verification of resistant cultivars.
亚洲稻瘿蚊对水稻产量构成严重威胁，因此培育抗稻瘿蚊水稻品种是防治稻瘿蚊最具成本效益和效率的策略。对115份水稻种质资源组成的多样化群体进行了表型分析，结果显示对稻瘿蚊生物型2的抗性存在差异。在进行全基因组关联研究以鉴定稻瘿蚊抗性的标记-性状关联（MTA）之前，评估了该群体的多样性和家族亲缘关系。新开发的候选基因衍生标记与随机微卫星标记一起用于基因分型。共发现50个P < 0.05的显著MTA。除第11号染色体外，所有水稻染色体均有显著的MTA。在第6、8和9号染色体上鉴定出的QTL与66F 67R、54F 55R和RM107相关，解释了最大的表型变异。相关标记的等位基因效应区分了易感和高抗基因型，证实了它们与稻瘿蚊抗性的关联。在稻瘿蚊抗性QTL区域发现了7个与一般胁迫耐受性反应相关的基因。在第9号染色体上，鉴定出一个推定的稻瘿蚊抗性基因，它与标记RM23914相关。鉴定出的这些候选基因对稻瘿蚊抗性反应有显著影响。本研究有助于更好地理解水稻抗稻瘿蚊机制，并为抗性品种的育种和功能验证提供重要的遗传信息。
