Shin Joo-Hee, Lee Dong-Yul, Lee Sung-Hoon
Department of Conservative Dentistry, Korea University Medical Center Guro Hospital, Republic of Korea.
Department of Orthodontics, Korea University Medical Center Guro Hospital, Seoul, Republic of Korea.
J Dent Sci. 2018 Mar;13(1):54-59. doi: 10.1016/j.jds.2017.10.007. Epub 2018 Feb 1.
BACKGROUND/PURPOSE: Bacterial infection is closely associated with the failure of endodontic treatment, and use of endodontic sealer with antimicrobial activity and biological compatibility is necessary for the success of root canal treatment. The purpose of this study was to investigate and to compare the antibacterial effect of two calcium silicate-based root canal sealers (Endoseal and EndoSequence BC sealer) as recent development sealers and with three conventional root canal sealers (AH Plus, Sealapex, and Tubli-Seal), before or after setting, on , and
The sealers were soaked in phosphate buffered saline to elute its compositions after and before setting, and the elutes were performed the antimicrobial assay. Also, X-ray fluorescence analysis was carried out to compare compositions of two calcium silicate-based sealers.
The conventional root canal sealers have strong antibacterial activity against the Gram-negative bacteria, and . Endoseal sealer showed antibacterial activity against not only the Gram-negative bacteria, but also against the Gram-positive bacteria, . However, Endosequence BC sealer exhibited a weak antibacterial effect on all bacteria in this study. X-ray fluorescence analysis exhibited that Endoseal contained more types and more amount of the oxide compound known to have strong antimicrobial activity such as AlO, FeO, MgO, NaO, NiO, and SO than Endoseqeunce BC.
Endoseal, which contains various types of oxide compounds, seems to be a suitable sealer for preventing bacterial infection in both treated and untreated root canals.
背景/目的：细菌感染与牙髓治疗失败密切相关，使用具有抗菌活性和生物相容性的牙髓封闭剂对于根管治疗的成功至关重要。本研究的目的是调查并比较两种新型硅酸钙基根管封闭剂（Endoseal和EndoSequence BC封闭剂）与三种传统根管封闭剂（AH Plus、Sealapex和Tubli-Seal）在凝固前后对[具体细菌名称未给出]的抗菌效果。
将封闭剂在凝固前后浸泡于磷酸盐缓冲盐水中以洗脱其成分，对洗脱液进行抗菌检测。此外，进行X射线荧光分析以比较两种硅酸钙基封闭剂的成分。
传统根管封闭剂对革兰氏阴性菌[具体细菌名称未给出]具有较强的抗菌活性。Endoseal封闭剂不仅对革兰氏阴性菌有抗菌活性，对革兰氏阳性菌[具体细菌名称未给出]也有抗菌活性。然而，EndoSequence BC封闭剂在本研究中对所有细菌的抗菌效果较弱。X射线荧光分析表明，Endoseal所含已知具有较强抗菌活性的氧化物化合物（如AlO、FeO、MgO、NaO、NiO和SO）的种类和含量均多于Endoseqeunce BC。
含有多种氧化物化合物的Endoseal似乎是预防已治疗和未治疗根管中细菌感染的合适封闭剂。
