Thakore Sanketsinh, Saini Rajat, Kushwaha Ravi Kant, K Asha, Thangavelu Indumathi, AlGhamdi AbdulAziz A, Tadepalli Srinivas
Department of Applied Science, PIT, Parul University, Vadodara, Gujarat, India.
Centre of Research Impact and Outcome, Chitkara University, Rajpura, 140417, Punjab, India.
Naunyn Schmiedebergs Arch Pharmacol. 2025 Jul 9. doi: 10.1007/s00210-025-04415-1.
HeLa cervical cancer cells exhibit high aggressiveness and proliferation, highlighting the need for novel therapies. Nanocomposite synthesis offers a promising approach due to its enhanced bioavailability, targeted delivery, and selective cytotoxicity. Thus, carboxymethyl cellulose (CMC) and D-carvone (Dcar)-coated strontium oxide (SrO) (SrO-CMC-Dcar) nanocomposite were synthesized using a wet chemical method. Structural and morphological characterization using XRD, FTIR, PL spectroscopy, DLS, HRTEM, and XPS confirmed the successful synthesis of the nanocomposite. XRD analysis revealed that the crystallite sizes of SrO and SrO-CMC-Dcar were 38 nm and 29 nm, respectively. PL spectra revealed prominent green emission at 516, 524, and 535 nm, indicating that oxygen vacancies are associated with ROS generation. DLS analysis revealed that pure SrO exhibited a particle size distribution of 128.7 nm, while the SrO-CMC-Dcar nanocomposite showed an increased size of 244.80 nm. The nanocomposite demonstrated an enhanced antimicrobial activity against MRSA and Candida albicans when compared to SrO alone. Furthermore, MTT assay results revealed that the SrO-CMC-Dcar composite significantly decreased HeLa cell viability to 11.94%, and the IC value was found to be 50.2 and 39.7 for SrO and nanocomposite SrO-CMC-Dcar, respectively, confirming it enhanced anticancer potential. In addition, the SrO-CMC-Dcar nanocomposite exhibited enhanced antioxidant properties demonstrated by DPPH free radical scavenging assays. These findings suggest that the SrO-CMC-Dcar nanocomposite holds promise for therapeutic applications in combating cancer, microbial infections, and oxidative stress.
人乳头瘤病毒18型（HeLa）宫颈癌细胞具有高度侵袭性和增殖能力，这凸显了对新型治疗方法的需求。纳米复合材料的合成因其提高的生物利用度、靶向递送和选择性细胞毒性而提供了一种有前景的方法。因此，采用湿化学法合成了羧甲基纤维素（CMC）和D-香芹酮（Dcar）包覆的氧化锶（SrO）（SrO-CMC-Dcar）纳米复合材料。使用X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、光致发光（PL）光谱、动态光散射（DLS）、高分辨率透射电子显微镜（HRTEM）和X射线光电子能谱（XPS）进行的结构和形态表征证实了纳米复合材料的成功合成。XRD分析表明，SrO和SrO-CMC-Dcar的微晶尺寸分别为38纳米和29纳米。PL光谱显示在516、524和535纳米处有明显的绿色发射，表明氧空位与活性氧生成有关。DLS分析表明，纯SrO的粒径分布为128.7纳米，而SrO-CMC-Dcar纳米复合材料的粒径增大至244.80纳米。与单独的SrO相比，该纳米复合材料对耐甲氧西林金黄色葡萄球菌（MRSA）和白色念珠菌表现出增强的抗菌活性。此外，噻唑蓝（MTT）测定结果显示，SrO-CMC-Dcar复合材料使HeLa细胞活力显著降低至11.94%，SrO和纳米复合材料SrO-CMC-Dcar的半数抑制浓度（IC）值分别为50.2和39.7，证实了其增强的抗癌潜力。此外，通过二苯基苦味酰基自由基（DPPH）清除试验证明，SrO-CMC-Dcar纳米复合材料具有增强的抗氧化性能。这些发现表明，SrO-CMC-Dcar纳米复合材料在对抗癌症、微生物感染和氧化应激的治疗应用中具有前景。
