Toshmatov Zokir O, Melikuziev Fazliddin A, Aytenov Ilkham S, Isokulov Ma'ruf Z, Kahar Gulnaz, Bozorov Tohir A, Zhang Daoyuan
Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
Laboratory of Bioresources and Stress Biology, Institute of Genetics and Plants Experimental Biology, Uzbek Academy of Sciences, Yukori-Yuz, Kibray 111226, Tashkent Region, Uzbekistan.
Plants (Basel). 2025 Jul 4;14(13):2058. doi: 10.3390/plants14132058.
Plant species harbor diverse rhizospheric bacteria within their resilient root zones, serving as a valuable reservoir of bioactive microorganisms with strong potential for natural antifungal and plant growth-promoting applications. This study aimed to investigate the antagonistic potential of , isolated from in the Aral Sea region, Uzbekistan, against the fungal pathogen . Due to its strong antifungal activity, was selected for bioactive compound profiling. Methanolic extracts were fractionated via silica and Sephadex gel chromatography, followed by antifungal screening using the agar diffusion method. A highly active fraction (dichloromethane/methanol, 9:1) underwent further purification, yielding twelve antifungal sub-fractions. Mass spectrometry analysis across positive and negative ion modes identified 2475 metabolites, with polar solvents-particularly methanol-enhancing compound recovery. Refinement using -specific references identified six known antibiotics. Four pure compounds were isolated and structurally characterized using NMR: oleanolic acid, ursolic acid, cyclo-(Pro-Ser), and uracil. Their growth regulatory activity was assessed on , , triticale, and at concentrations of 5, 20, 100, and 500 mg L-1. All compounds negatively affected root growth in a concentration-dependent manner, especially in monocots. Interestingly, some treatments enhanced stem growth, particularly in . These findings indicate that produces diverse bioactive compounds with dual antifungal and plant growth-modulatory effects, highlighting its potential as a biocontrol agent and a source of natural bioactive compounds.
植物物种在其具有韧性的根际区域内蕴藏着多样的根际细菌，作为具有生物活性的微生物的宝贵储存库，在天然抗真菌和促进植物生长应用方面具有巨大潜力。本研究旨在调查从乌兹别克斯坦咸海地区的[具体植物名称]中分离出的[细菌名称]对真菌病原体[病原体名称]的拮抗潜力。由于其强大的抗真菌活性，[细菌名称]被选用于生物活性化合物分析。甲醇提取物通过硅胶和葡聚糖凝胶色谱进行分离，随后使用琼脂扩散法进行抗真菌筛选。一个高活性级分（二氯甲烷/甲醇，9:1）进一步纯化，得到12个抗真菌亚级分。在正离子和负离子模式下的质谱分析鉴定出2475种代谢物，极性溶剂（特别是甲醇）可提高化合物回收率。使用[具体参考物质]进行的精制鉴定出六种已知抗生素。分离出四种纯化合物并使用核磁共振进行结构表征：齐墩果酸、熊果酸、环（脯氨酸-丝氨酸）和尿嘧啶。在浓度为5、20、100和500 mg L-1时，评估了它们对[植物名称1]、[植物名称2]、小黑麦和[植物名称3]的生长调节活性。所有化合物均以浓度依赖的方式对根生长产生负面影响，尤其是在单子叶植物中。有趣的是，一些处理增强了茎的生长，特别是在[植物名称4]中。这些发现表明，[细菌名称]产生具有抗真菌和植物生长调节双重作用的多种生物活性化合物，突出了其作为生物防治剂和天然生物活性化合物来源的潜力。
