Ma Hongxue, Deng Siping, Kong Congcong, Zhang Yulun, Zhao Tong, Ji Jialei, Wang Yong, Zhang Yangyong, Zhuang Mu, Yang Limei, Lebedeva Marina, Taranov Vasiliy, Artemyeva Anna M, Fang Zhiyuan, Yu Jingquan, Hu Zhangjian, Lv Honghao
Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2025 Jun 26;26(13):6179. doi: 10.3390/ijms26136179.
4-coumarate-CoA ligase (4CL) plays a crucial role in the phenylpropanoid metabolic pathway and is a key enzyme involved in plant growth and stress responses. Black rot, caused by pv. () is a major bacterial disease affecting the production of global cruciferous crop-like cabbage ( var. ). However, the role of genes in cabbage resistance to black rot remains unclear. In this study, transcriptome sequencing was conducted using resistant cabbage MY and susceptible cabbage LY at 0, 6, 24, and 48 h post-inoculation. KEGG analysis identified the enrichment of the phenylpropanoid biosynthesis pathway, and significant expression changes of genes were determined through the expression heat map. Further genome-wide analysis revealed 43 gene family members on the cabbage genome distributed across nine chromosomes. Gene structure and protein motif analysis revealed similarities in motifs within the same evolutionary branch, but variations in gene structure. A combination of gene expression profiles and differentially expressed genes (DEGs) from the transcriptome identified as a key gene for further study. Inoculation of overexpressed T generation stably expressed cabbage seedlings demonstrated significantly larger lesion areas compared to wild type cabbage, indicating that negatively regulates resistance to black rot in cabbage. The analysis of multi-time point transcriptomes in cabbage and the functional study of the gene family enhance our understanding of the mechanisms underlying plant disease resistance. This provides compelling evidence and experimental support for elucidating the mechanisms of black rot resistance in cabbage.
4-香豆酸辅酶A连接酶（4CL）在苯丙烷类代谢途径中起着关键作用，是参与植物生长和应激反应的关键酶。由野油菜黄单胞菌野油菜致病变种（Xanthomonas campestris pv. campestris）引起的黑腐病是影响全球十字花科作物如甘蓝（Brassica oleracea var. capitata）产量的主要细菌性病害。然而，4CL基因在甘蓝对黑腐病抗性中的作用仍不清楚。在本研究中，对接种后0、6、24和48小时的抗性甘蓝MY和感病甘蓝LY进行了转录组测序。KEGG分析确定了苯丙烷类生物合成途径的富集，并通过表达热图确定了4CL基因的显著表达变化。进一步的全基因组分析揭示了甘蓝基因组上分布在9条染色体上的43个4CL基因家族成员。基因结构和蛋白质基序分析揭示了同一进化分支内基序的相似性，但基因结构存在差异。结合4CL基因表达谱和转录组中的差异表达基因（DEG）确定了一个关键基因以供进一步研究。接种过表达4CL的T代稳定表达甘蓝幼苗，与野生型甘蓝相比，其病斑面积显著更大，表明4CL负向调节甘蓝对黑腐病的抗性。对甘蓝多时间点转录组的分析以及4CL基因家族的功能研究增强了我们对植物抗病机制的理解。这为阐明甘蓝抗黑腐病机制提供了有力证据和实验支持。
