Sulaiman Seerwan Hamadameen, Ali Hoshyar Saadi, Omer Rebaz Anwar, Barzani Hemn A H, Salih Musher Ismael, Qader Aryan Fathulla
Department of Medical Laboratory Science, College of Health Science, Lebanese French University, Erbil, Iraq.
Department of Medical Microbiology, College of Science, Knowledge University, Kirkuk, Erbil, Iraq.
Cell Biochem Biophys. 2025 Jul 16. doi: 10.1007/s12013-025-01827-x.
The purpose of this research was to assess oxidative stress levels in colon cancer patients and examine their association with disease onset and progression. 176 individuals were recruited, comprising 106 colon cancer patients and 70 healthy controls. Serum oxidative stress marker levels of protein carbonyl (PCO), ischemia-modified albumin (IMA), malondialdehyde (MDA), and glutathione-S-transferase (GST) and antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) were quantified. The tumor markers carcinoembryonic antigen (CEA) and cancer antigen 19-9 (CA 19.9) were also evaluated. The levels of PCO, IMA, MDA, and GST were significantly increased (P < 0.01 for each) with a significant decrease in GPX and SOD levels (P < 0.01) when compared to the control group. No significant difference was noted in CAT levels. The tumor markers CEA and CA 19.9 were significantly increased in the patient group (P < 0.01). These results suggest an imbalance of oxidative/antioxidant status in favor of oxidative stress in patients with colon cancer. The study identifies oxidative stress as a major factor in the pathogenesis of colon cancer. Clinically, biomarkers such as IMA with more than 80% sensitivity can be powerful secondary aids to early detection or monitoring disease progression. The findings suggest that modulating oxidative stress would be therapeutic in the treatment of colon cancer.
本研究的目的是评估结肠癌患者的氧化应激水平，并探讨其与疾病发生和进展的关联。招募了176名个体，包括106名结肠癌患者和70名健康对照者。对血清氧化应激标志物蛋白羰基（PCO）、缺血修饰白蛋白（IMA）、丙二醛（MDA）和谷胱甘肽-S-转移酶（GST）以及抗氧化酶超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）和过氧化氢酶（CAT）的水平进行了定量分析。还评估了肿瘤标志物癌胚抗原（CEA）和癌抗原19-9（CA 19.9）。与对照组相比，PCO、IMA、MDA和GST的水平显著升高（各P<0.01），而GPX和SOD的水平显著降低（P<0.01）。CAT水平未观察到显著差异。患者组的肿瘤标志物CEA和CA 19.9显著升高（P<0.01）。这些结果表明，结肠癌患者的氧化/抗氧化状态失衡，有利于氧化应激。该研究确定氧化应激是结肠癌发病机制中的一个主要因素。临床上，敏感性超过80%的生物标志物如IMA可作为早期检测或监测疾病进展的有力辅助手段。研究结果表明，调节氧化应激在结肠癌治疗中具有治疗作用。
