Nyasulu Mvuyeni, Zhong Qi, Wang Zhengjie, Cheng Zhicheng, Zhihao Chen, Yang Jun, He Haohua, Bian Jianmin
The Joint Laboratory of International Cooperation in Crop Genetic Improvement of Multiple Cropping System of Ministry of Education, Nanchang, 330045, China.
College of Agronomy, Jiangxi Agricultural University, Nanchang, China.
Rice (N Y). 2025 Jul 21;18(1):71. doi: 10.1186/s12284-025-00828-x.
This study presents a detailed analysis of the molecular mechanisms involved in heat stress tolerance in rice, focusing on the endoplasmic reticulum (ER) protein processing pathway. Through RNA sequencing (RNA-seq), we identified differentially expressed genes in two rice varieties, BNP162 and BNP206, emphasizing the importance of ER quality control mechanisms in maintaining cellular balance during heat stress. We identified three novel genes, Os11g0244200, Os01g0135800, and Os04g0445100, belonging to the Hsp20/alpha crystallin family, which are upregulated in response to heat stress. These genes play essential roles in protein stabilization, folding, and preventing aggregation, critical functions for maintaining protein balance under stress conditions. The upregulation of these genes highlights their potential in enhancing thermotolerance, a key trait for rice cultivation in the face of global climate change challenges. Our findings suggest that these novel genes could be promising targets for genetic manipulation to enhance heat tolerance in rice, contributing to the development of heat-resistant rice varieties. This research provides new insights into the molecular mechanisms of heat stress adaptation and lays a solid foundation for future studies aimed at improving crop resilience to environmental stress.
本研究对水稻耐热性所涉及的分子机制进行了详细分析，重点关注内质网（ER）蛋白加工途径。通过RNA测序（RNA-seq），我们在两个水稻品种BNP162和BNP206中鉴定出差异表达基因，强调了内质网质量控制机制在热胁迫期间维持细胞平衡中的重要性。我们鉴定出三个属于Hsp20/α晶状体蛋白家族的新基因，即Os11g0244200、Os01g0135800和Os04g0445100，它们在热胁迫下上调。这些基因在蛋白质稳定、折叠以及防止聚集方面发挥着重要作用，这些功能对于在胁迫条件下维持蛋白质平衡至关重要。这些基因的上调突出了它们在增强耐热性方面的潜力，耐热性是面对全球气候变化挑战时水稻种植的关键性状。我们的研究结果表明，这些新基因可能是通过基因操作提高水稻耐热性的有前景的靶点，有助于培育耐热水稻品种。本研究为热胁迫适应的分子机制提供了新见解，并为未来旨在提高作物对环境胁迫的耐受性的研究奠定了坚实基础。
