Sobh Rokaya A, Magar Hend S
Polymers and Pigments Department, National Research Centre, Dokki, P.O. Box 12622, Giza, Egypt.
Applied Organic Chemistry Department, National Research Centre, Dokki, P.O. Box 12622, Giza, Egypt.
Nanoscale. 2025 Aug 7;17(31):18359-18376. doi: 10.1039/d5nr01765c.
The detection of folic acid in food samples or pharmaceutical drugs is crucial since folic acid affects the development and growth of a fetus and the biological processes of the human body. In this study, we aimed to develop an advanced multifunctional electrochemical sensor for folic acid detection in pharmaceutical and food samples utilizing a synthetic functional copolymer at the nanoscale. The multifunctional copolymer nanospheres [P(MMA/GMA/AN)] prepared from methyl methacrylate (MMA), glycidyl methacrylate (GMA), and acrylonitrile (AN) were synthesized using an environmentally friendly free radical microemulsion polymerization method. The distinctive high-functional copolymer nanospheres were analyzed through Fourier-transform infrared (FT-IR) spectroscopy, thermogravimetric (TG) analysis, and transmission electron microscopy (TEM) and compared with those of the respective homopolymers PMMA, PGMA and PAN. The electrochemical characterizations of P(MMA/GMA/AN) and its respective homopolymers (PMMA, PGMA and PAN) were evaluated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The (MMA/GMA/AN) terpolymer demonstrated markedly superior electrical conductivity when compared with that of the homopolymers, which were ranked in terms of electrical conductivity values as follows: PAN, PGMA, and PMMA. Furthermore, a copolymer-modified screen-printed electrode (SPE) sensor successfully detected the presence of folic acid using differential pulse voltammetry (DPV) with a wide linear range from 0.1 nM to 5000 nM and a limit of detection of 0.015 nM. The sensor was applied for FA detection in food samples and pharmaceutical products with high recovery.
食品样品或药品中叶酸的检测至关重要，因为叶酸会影响胎儿的发育和生长以及人体的生物过程。在本研究中，我们旨在利用纳米级合成功能共聚物开发一种先进的多功能电化学传感器，用于检测药品和食品样品中的叶酸。由甲基丙烯酸甲酯（MMA）、甲基丙烯酸缩水甘油酯（GMA）和丙烯腈（AN）制备的多功能共聚物纳米球[P（MMA/GMA/AN）]采用环境友好的自由基微乳液聚合方法合成。通过傅里叶变换红外（FT-IR）光谱、热重（TG）分析和透射电子显微镜（TEM）对独特的高功能共聚物纳米球进行了分析，并与相应的均聚物聚甲基丙烯酸甲酯（PMMA）、聚甲基丙烯酸缩水甘油酯（PGMA）和聚丙烯腈（PAN）进行了比较。使用循环伏安法（CV）和电化学阻抗谱（EIS）对P（MMA/GMA/AN）及其相应的均聚物（PMMA、PGMA和PAN）进行了电化学表征。与均聚物相比，三元共聚物（MMA/GMA/AN）表现出明显优异的电导率，其电导率值排序如下：PAN、PGMA和PMMA。此外，一种共聚物修饰的丝网印刷电极（SPE）传感器使用差分脉冲伏安法（DPV）成功检测到叶酸的存在，线性范围宽，从0.1 nM到5000 nM，检测限为0.015 nM。该传感器应用于食品样品和药品中叶酸的检测，回收率高。
