Meepun Nipaporn, Sommart Ubonta, Bualuang Oraporn
Chemistry Program, Faculty of Science and Technology, Suratthani Rajabhat University, Surat Thani, 84100, Thailand.
J Cannabis Res. 2025 Jul 26;7(1):50. doi: 10.1186/s42238-025-00309-4.
With the growing interest in the therapeutic applications of L., understanding how extraction techniques influence its antioxidant potential and phytochemical composition is essential for optimizing product quality and functionality.
This study evaluated the impact of various oil-based extraction methods—specifically using medium-chain triglycerides (MCT)—on the antioxidant activity and cannabinoid content of sugar leaf, leaf, and root. The extraction techniques investigated included ultrasound-assisted extraction (UAE), heated infusion extraction (IE), ultrasound-assisted infusion extraction (UIE), and microwave-assisted extraction (MAE).
Significant differences were observed in the physicochemical and antioxidant properties of the extracted oils. Compared to UAE, the IE, UIE, and MAE methods resulted in 26.4–148.3% higher color intensity. Peroxide values ranged from 0.055 to 0.276 mg O₂ eq/kg, indicating acceptable oxidative stability. Total phenolic content varied between 2.16 and 125.27 mg gallic acid equivalents (GAE)/100 g, with IE of sugar leaf yielding 131.8% higher phenolics than root extracts. Antioxidant assays revealed that IE-extracted sugar leaf oil showed superior activity, with increases of 3.16-fold (DPPH), 0.32-fold (ABTS), 6.18-fold (FRAP), and 3.89-fold (1,10-phenanthroline) compared to UAE. Furthermore, the IE method yielded the highest levels of CBD (0.027 g/100 g dry matter) and THC (0.746 g/100 g dry matter) in sugar leaf extracts.
These findings highlight the sugar leaf as a valuable by-product rich in bioactive compounds. Heated infusion extraction notably enhances the antioxidant and cannabinoid content of cannabis oils, offering potential for nutraceutical and cosmetic applications.
随着人们对[植物名称]治疗应用的兴趣日益浓厚，了解提取技术如何影响其抗氧化潜力和植物化学成分对于优化产品质量和功能至关重要。
本研究评估了各种油基提取方法——特别是使用中链甘油三酯（MCT）——对糖叶、叶片和根部的抗氧化活性和大麻素含量的影响。所研究的提取技术包括超声辅助提取（UAE）、加热浸提（IE）、超声辅助浸提（UIE）和微波辅助提取（MAE）。
在提取油的物理化学和抗氧化性能方面观察到显著差异。与UAE相比，IE、UIE和MAE方法导致颜色强度高26.4 - 148.3%。过氧化值范围为0.055至0.276毫克O₂当量/千克，表明氧化稳定性可接受。总酚含量在2.16至125.27毫克没食子酸当量（GAE）/100克之间变化，糖叶的IE提取物中的酚类物质比根部提取物高131.8%。抗氧化分析表明，IE提取的糖叶油表现出优异的活性，与UAE相比，DPPH增加3.16倍、ABTS增加0.32倍、FRAP增加6.18倍、1,10 - 菲啰啉增加3.89倍。此外，IE方法在糖叶提取物中产生了最高水平的CBD（0.027克/100克干物质）和THC（0.746克/100克干物质）。
这些发现突出了糖叶作为富含生物活性化合物的有价值副产品。加热浸提显著提高了大麻油的抗氧化和大麻素含量，为营养保健品和化妆品应用提供了潜力。
