从纳豆芽孢杆菌中分离和鉴定四种类型的质粒。
Isolation and characterization of four types of plasmids from Bacillus subtilis (natto).
作者信息
Tanaka T, Koshikawa T
出版信息
J Bacteriol. 1977 Aug;131(2):699-701. doi: 10.1128/jb.131.2.699-701.1977.
Covalently closed circular deoxyribonucleic acids were found in 10 strains of Bacillus natto. The plasmids could be classified into four types on the basis of the molecular weights as well as the patterns in agarose gel electrophoresis after digestion with restriction endonucleases: (i) plasmids (seven were detected) with a molecular weight of 3.6 X 10(6); (ii) plasmids (two were detected) with a molecular weight of 4.0 X 10(6); (iii) plasmids (eight were detected) with a molecular weight of about 34 X 10(6); and (iv), a plasmid with an approximate molecular weight of 46 X 10(6). Out of the 10 plasmid-carrying strains, 6 (IFO3009, IFO3013, IFO3335, IFO13169, IAM1143, and IAM1207) harbored both type 1 and 3 plasmids; 2 (IAM1114 and IAM1168) harbored both type 2 and 3 plasmids, and IFO3936 and IAM1163 carried type 1 and 4 plasmids, respectively.
在10株纳豆芽孢杆菌中发现了共价闭合环状脱氧核糖核酸。根据分子量以及用限制性内切酶消化后在琼脂糖凝胶电泳中的图谱，这些质粒可分为四种类型：（i）分子量为3.6×10⁶的质粒（检测到7个）；（ii）分子量为4.0×10⁶的质粒（检测到2个）；（iii）分子量约为34×10⁶的质粒（检测到8个）；以及（iv）分子量约为46×10⁶的质粒。在10个携带质粒的菌株中，6个（IFO3009、IFO3013、IFO3335、IFO13169、IAM1143和IAM1207）同时携带1型和3型质粒；2个（IAM1114和IAM1168）同时携带2型和3型质粒，IFO3936和IAM1163分别携带1型和4型质粒。
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