Raps Felix C, Hyster Todd K
Department of Chemistry, Princeton University, Princeton, New Jersey 08544 United States.
ACS Cent Sci. 2025 Jun 5;11(7):1029-1040. doi: 10.1021/acscentsci.5c00245. eCollection 2025 Jul 23.
Enzymes are invaluable tools for solving challenges in synthetic organic chemistry. Beyond replicating native reactivity patterns, modern directed evolution strategies have enabled chemists to efficiently survey chemical space to identify enzyme families capable of catalyzing non-natural reactions. While methods often focus on chemo-, enantio-, and regiocontrol, there are a growing number of examples that describe reactivity patterns and reaction mechanisms that were previously unknown in the synthetic literature. In this Perspective, we will explore examples of such emergent mechanistic pathways of enzymes in the context of synthetic precedents, emphasizing the remarkable versatility of diverse enzyme active sites in controlling unprecedented transformations.
酶是解决合成有机化学难题的宝贵工具。除了复制天然反应模式外，现代定向进化策略使化学家能够有效地探索化学空间，以识别能够催化非天然反应的酶家族。虽然方法通常侧重于化学、对映体和区域控制，但越来越多的例子描述了合成文献中以前未知的反应模式和反应机制。在本综述中，我们将在合成先例的背景下探讨酶的此类新兴机制途径的例子，强调不同酶活性位点在控制前所未有的转化方面的显著通用性。
