Sánchez-Argáez Ana Beatriz, Herrera-Torres Estefania, Moreno-Lafont Martha Cecilia, Flores-Romo Leopoldo, López-Santiago Rubén
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. de Carpio y Plan de Ayala s/n, Del. Miguel Hidalgo, Ciudad de México 11340, Mexico.
Departamento de Biomedicina Molecular y Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados (CINVESTAV), Instituto Politécnico Nacional, Av. IPN No. 2508. Del. Gustavo A. Madero, Ciudad de México 07360, Mexico.
Microorganisms. 2025 Jun 20;13(7):1442. doi: 10.3390/microorganisms13071442.
The main aspects of brucellosis have been studied in animal models to better understand the pathogenesis of the disease. Mice are the most common animal model of brucellosis. To verify that the infection has been successfully induced, it is necessary to assess the presence of in experimentally infected mice. Traditionally, high doses of have been used to establish detectable infection in oral murine models but prevent the emulation of natural pathogenesis. We propose the use of a low dose (1 × 10 CFUs) to establish a more realistic oral infection model. Using a two-step procedure consisting of selective broth enrichment followed by agar isolation, we were able to recover bacteria from gut-associated lymphoid tissues (mesenteric lymph nodes and Peyer's patches), the spleen, and feces during the early and late stages of infection (1 h and up to 5 weeks). This technique promotes the study of early infection stages and systemic dissemination without the need for high doses to induce infection orally. It also demonstrates that remains in the intestinal-associated lymphoid tissues at time points when the infection is already systemically established.
为了更好地理解布鲁氏菌病的发病机制，已在动物模型中对该病的主要方面进行了研究。小鼠是布鲁氏菌病最常用的动物模型。为了验证是否已成功诱导感染，有必要评估实验感染小鼠体内是否存在（此处原文缺失关键信息）。传统上，在口服小鼠模型中使用高剂量（此处原文缺失关键信息）来建立可检测到的感染，但这无法模拟自然发病机制。我们建议使用低剂量（1×10菌落形成单位）来建立更符合实际的口服感染模型。通过两步法，即先进行选择性肉汤富集，然后进行琼脂分离，我们能够在感染的早期和晚期（1小时至长达5周）从肠道相关淋巴组织（肠系膜淋巴结和派尔集合淋巴结）、脾脏和粪便中分离出细菌。该技术有助于研究感染的早期阶段和全身播散，而无需高剂量口服诱导感染。它还表明，在感染已全身确立的时间点，（此处原文缺失关键信息）仍存在于肠道相关淋巴组织中。
