Garcia-Salazar Karla S, Leon-Solano Hector J, Maguey-Gonzalez Jesus A, Latorre Juan D, López-Arellano Raquel, Morales Hipólito Elvia A, Díaz-Torres Roberto, Morales Rodríguez Miguel, Vázquez-Durán Alma, Tellez-Isaias Guillermo, Méndez-Albores Abraham, Solis-Cruz Bruno, Hernandez-Patlan Daniel
Nanotechnology Engineering Division, Polytechnic University of the Valley of Mexico, Tultitlan 54910, Mexico.
Division of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.
Pharmaceutics. 2025 Jun 27;17(7):840. doi: 10.3390/pharmaceutics17070840.
Infantis (. Infantis) is a bacterium that has gained importance in public health over the last decade due to its high pathogenicity and resistance to antibiotics. Therefore, the objective of the present study was to present key considerations for the design and development of geraniol-loaded nanocapsules for its delivery in the drinking water or feed of broiler chickens and to evaluate its potential as an antimicrobial agent against . Infantis using a standard in vitro microplate assay and a model that simulates the pH and feed conditions of the crop of broiler chickens. : Using a 3 factorial experimental design, geraniol nanocapsule-based formulations were selected, and their antimicrobial activity was evaluated in in vitro models. : The results demonstrated that geraniol alone exhibits antimicrobial action against . Infantis mainly due to its lipophilicity, hydrophobicity and the presence of the hydroxyl group found in its chemical structure, but when formulated in nanocapsular systems, the interaction of its components tends to reduce its antimicrobial action, especially the mixture of Tween 80:Span 80 and Miglyol 810N. Furthermore, the use of the in vitro model that simulates the crop of broiler chickens demonstrated that the formulation also has interactions with the feed components, completely nullifying the antimicrobial action of geraniol compared to that obtained in the in vitro microplate model. : Preformulation studies during the development of nanocapsule-based formulations should be considered for the correct selection of the components of a formulation to ensure its effectiveness, without only considering the physicochemical and stability properties of these as is frequently seen in studies.
婴儿双歧杆菌（. Infantis）是一种细菌，在过去十年中因其高致病性和抗生素耐药性而在公共卫生领域变得愈发重要。因此，本研究的目的是提出关于设计和开发负载香叶醇的纳米胶囊的关键考虑因素，以便将其添加到肉鸡的饮用水或饲料中，并使用标准体外微孔板试验和模拟肉鸡嗉囊pH值及饲料条件的模型来评估其作为抗婴儿双歧杆菌抗菌剂的潜力。：采用三因素实验设计，选择了基于香叶醇纳米胶囊的制剂，并在体外模型中评估了它们的抗菌活性。：结果表明，香叶醇单独使用时对婴儿双歧杆菌具有抗菌作用，这主要归因于其亲脂性、疏水性以及化学结构中存在的羟基，但当制成纳米胶囊系统时，其成分之间的相互作用往往会降低其抗菌作用，尤其是吐温80:司盘80和Miglyol 810N的混合物。此外，使用模拟肉鸡嗉囊的体外模型表明，该制剂还与饲料成分相互作用，与在体外微孔板模型中获得的结果相比，完全消除了香叶醇的抗菌作用。：在基于纳米胶囊的制剂开发过程中，应进行处方前研究，以便正确选择制剂的成分以确保其有效性，而不仅仅像在研究中常见的那样只考虑这些成分的物理化学和稳定性特性。
