Costa Bárbara, Pereira Eurico, Ferreira-Filho Vital C, Pires Ana Salomé, Pereira Laura C J, Soares Paula I P, Botelho Maria Filomena, Mendes Fernando, Graça Manuel P F, Teixeira Sílvia Soreto
i3N, Physics Department, University of Aveiro, 3810-193 Aveiro, Portugal.
Coimbra Institute for Clinical and Biomedical Research (iCBR) Area of Environment Genetics and Oncobiology (CIMAGO), Institute of Biophysics, Faculty of Medicine, Universidade de Coimbra, Pólo III-Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal.
Pharmaceutics. 2025 Jun 27;17(7):844. doi: 10.3390/pharmaceutics17070844.
Nanotechnology, specifically magnetic nanoparticles (MNPs), is revolutionizing cancer treatment. Magnetic hyperthermia is a treatment that, using MNPs, can selectively kill cancer cells without causing damage to the surrounding tissues. : This work aimed to analyze how the synthesis conditions, namely, how the pH of the reaction can influence the magnetic properties of FeO nanoparticles for magnetic hyperthermia, using the hydrothermal synthesis. : For the hydrothermal synthesis, FeCl·6HO and FeCl·4HO were mixed with different quantities of NaOH to adjust the pH. After obtaining a black precipitate, the samples were placed in an autoclave at 200 °C for 60 h, followed by a washing and drying phase. The obtained MNPs were analyzed using X-Ray Diffraction (XRD), Transmission Electron Microscopy, a Superconducting Quantum Interference Device, Specific Absorption Rate analysis, and cytotoxicity assays. : Different MNPs were analyzed (9.06 < pH < 12.75). The XRD results showed the presence of various iron oxide phases (magnetite, maghemite, and hematite), resulting from the oxidization of the iron phases present in the autoclave. In terms of the average particle size, it was verified that, by increasing the pH value, the size decreases (from 53.53 nm to 9.49 nm). Additionally, MNPs possess a superparamagnetic behaviour with high SAR values (above 69.3 W/g). : It was found that the pH of the reaction can influence the size, morphology, magnetization, and thermal efficiency of the MNP. The MNP with the highest composition of FeO was synthesized with a pH of 12.75, with a cubic morphology and a SAR value of 92.7 ± 3.2 W/g.
纳米技术，特别是磁性纳米颗粒（MNPs），正在彻底改变癌症治疗方式。磁热疗是一种利用MNPs选择性杀死癌细胞而不损伤周围组织的治疗方法。 ：这项工作旨在分析合成条件，即反应的pH值如何通过水热合成影响用于磁热疗的FeO纳米颗粒的磁性。 ：对于水热合成，将FeCl·6HO和FeCl·4HO与不同量的NaOH混合以调节pH值。获得黑色沉淀后，将样品置于200℃的高压釜中60小时，随后进行洗涤和干燥阶段。使用X射线衍射（XRD）、透射电子显微镜、超导量子干涉装置、比吸收率分析和细胞毒性测定对所得MNPs进行分析。 ：分析了不同的MNPs（9.06 < pH < 12.75）。XRD结果显示存在各种氧化铁相（磁铁矿、磁赤铁矿和赤铁矿），这是由高压釜中存在的铁相氧化产生的。就平均粒径而言，证实通过增加pH值，粒径减小（从53.53 nm减小到9.49 nm）。此外，MNPs具有高比吸收率值（高于69.3 W/g）的超顺磁行为。 ：发现反应的pH值会影响MNP的尺寸、形态、磁化和热效率。FeO组成最高的MNP是在pH值为12.75的条件下合成的，具有立方形态，比吸收率值为92.7 ± 3.2 W/g。
