Wauters G, Goossens V, Janssens M, Vandepitte J
Microbiology Unit, University of Louvain, Brussels, Belgium.
Appl Environ Microbiol. 1988 Apr;54(4):851-4. doi: 10.1128/aem.54.4.851-854.1988.
A new enrichment medium for the recovery of pathogenic Yersinia enterocolitica serogroup O:3 from naturally infected meat products based on three selective agents, Irgasan, ticarcillin, and potassium chlorate (ITC), was compared with several other one- or two-step enrichments. Y. enterocolitica serogroup O:3 was recovered from 96.5% of 29 pork tongues, 24% of 50 ground pork samples, 16% of 25 masseter muscle samples, and 61% of tonsils. ITC was by far the most sensitive method for the recovery of Y. enterocolitica O:3, especially from ground meat and masseter muscles, while cold and two-step enrichments yielded better results for nonpathogenic strains. Plating of ITC enrichments onto SS-deoxycholate-calcium agar gave overall better results than plating onto cefsulodin-Irgasan-novobiocin agar for serogroup O:3.
一种基于三种选择剂（三氯生、替卡西林和氯酸钾，即ITC）从自然感染的肉制品中分离致病性小肠结肠炎耶尔森氏菌O:3血清型的新型增菌培养基，与其他几种单步或两步增菌方法进行了比较。从29个猪舌中的96.5%、50个绞碎猪肉样品中的24%、25个咬肌样品中的16%以及扁桃体中的61%中分离出小肠结肠炎耶尔森氏菌O:3血清型。ITC是目前分离小肠结肠炎耶尔森氏菌O:3血清型最灵敏的方法，尤其是从绞碎肉和咬肌中分离时，而冷增菌和两步增菌法对非致病性菌株的分离效果更好。对于O:3血清型，将ITC增菌液接种到SS-脱氧胆酸钙琼脂上的总体效果优于接种到头孢磺啶-三氯生-新生霉素琼脂上。
