Talan M I, Ingram D K
Arch Gerontol Geriatr. 1985 Oct;4(3):251-9. doi: 10.1016/0167-4943(85)90007-x.
At 6 mth of age, male C57BL/6J mice were subjected to an intermittent schedule of feeding (every-other-day) or continued to be maintained on an ad libitum diet (24% protein). This regimen of dietary restriction resulted in increased mean and maximum lifespan (11%) compared to the survival of ad libitum-fed mice. At 8 and 26 mth of age, different groups of mice were tested for cold tolerance during 3 h exposure to 10 degrees C. Aged mice (26 mth) fed ad libitum compared to young mice (8 mth) on the same diet had significantly lower baseline colonic temperatures prior to cold exposure, and impaired cold tolerance, as measured by the rate of decline in colonic temperature during cold exposure. Dietary restriction by intermittent feeding had no significant effect on cold tolerance in young mice or baseline colonic temperature in young or aged mice. However, the cold tolerance of aged mice subjected to 20 mth of intermittent feeding was markedly improved over that of aged cohorts maintained on an ad libitum diet and in fact was indistinguishable from that of young counterparts. Thus, it appeared that this regimen of dietary restriction when implemented in young adults prevented the age-related decline in cold tolerance observed among ad libitum fed mice of this strain.
6月龄时，雄性C57BL/6J小鼠接受间歇性喂食方案（隔天喂食），或继续自由采食（24%蛋白质）。与自由采食小鼠的存活率相比，这种饮食限制方案使平均寿命和最长寿命增加了11%。在8月龄和26月龄时，对不同组的小鼠进行了测试，让它们在10摄氏度环境下暴露3小时，以检测其耐寒性。与食用相同饮食的年轻小鼠（8月龄）相比，自由采食的老年小鼠（26月龄）在冷暴露前的结肠基线温度显著较低，并且耐寒性受损，这通过冷暴露期间结肠温度的下降速率来衡量。间歇性喂食造成的饮食限制对年轻小鼠的耐寒性或年轻及老年小鼠的结肠基线温度没有显著影响。然而，经过20个月间歇性喂食的老年小鼠的耐寒性比自由采食的老年小鼠组有显著改善，事实上与年轻小鼠的耐寒性没有区别。因此，似乎这种饮食限制方案在年轻成年小鼠中实施时，可防止该品系自由采食小鼠中观察到的与年龄相关的耐寒性下降。
