Giaquinto C, Vanin M, Anglani F, Errico G, Ruga E, D'Elia R
Infection. 1985 Nov-Dec;13(6):260-2. doi: 10.1007/BF01645434.
A simple and rapid staphylococcal coagglutination test, using rabbit antisera prepared against Nebraska calf diarrhea virus (NCDV), is described for the detection of rotavirus in neonatal fecal specimens. When the samples were examined directly using the coagglutination test, more than 60% of the specimens agglutinated the control reagent. These non-specific reactions were markedly reduced by preincubation of the specimens with non-immune rabbit serum and further heating at 80 degrees C for 45 min. Such treatment did not reduce the specific activity in the coagglutination test when rotavirus-containing stools were tested. The coagglutination test was compared with ELISA in 290 stools positive or negative for rotavirus. The sensitivity of the coagglutination test was 92%, the specificity 91% and the predictive value 31%. These results indicate that coagglutination is a suitable test for rapid screening of rotavirus infection in clinical practice.
本文描述了一种简单快速的葡萄球菌协同凝集试验，该试验使用针对内布拉斯加犊牛腹泻病毒（NCDV）制备的兔抗血清，用于检测新生儿粪便标本中的轮状病毒。当直接使用协同凝集试验检测样本时，超过60%的标本凝集了对照试剂。通过将标本与非免疫兔血清预孵育并在80℃进一步加热45分钟，这些非特异性反应显著减少。当检测含轮状病毒的粪便时，这种处理并未降低协同凝集试验的特异性活性。对290份轮状病毒阳性或阴性的粪便进行了协同凝集试验与ELISA的比较。协同凝集试验的敏感性为92%，特异性为91%，预测值为31%。这些结果表明，协同凝集试验是临床实践中快速筛查轮状病毒感染的合适检测方法。
