Zhang Jing, Mou Tong, Li Cong-Jian, Bai Jing-Lin, Yu Li-Yan, Chen Hua-Hong, Zhang Yu-Qin
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
College of Resources, Environmental Sciences and Chemistry, Chuxiong Normal University, Chuxiong, Yunnan, 675000, PR China.
Int J Syst Evol Microbiol. 2025 Aug;75(8). doi: 10.1099/ijsem.0.006855.
Four Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterial strains (CPCC 206391, CPCC 206453, CPCC 206435 and CPCC 206450) were isolated from soil samples collected from Tibet Autonomous Region, China. The 16S rRNA gene sequences of these four strains showed close relations to members of the genus of the family , with similarities of 96.1-99.4% to the validly named species of . In the phylogenetic trees based on 16S rRNA gene sequences and the core genome, these isolates clustered into the genus clade within the lineage of the family . Genome relatedness index values between these strains and their phylogenetic neighbours, including average nucleotide identity (78.1-91.5%) and digital DNA-DNA hybridization (19.8-41.0%), were all below the species delineation thresholds. These genomic data, combined with their phenotypic characteristics, supported their classification within the genus , representing four novel species. Thereby, sp. nov. (type strain CPCC 206391=XZ253=KCTC 59139), sp. nov. (type strain CPCC 206453=CXZ644=KCTC 59274), sp. nov. (type strain CPCC 206435=CXZ904=KCTC 59271) and sp. nov. (type strain CPCC 206450=XZ100=KCTC 59273) were proposed. These strains exhibited stable growth under high-intensity UV radiation, attributed to the presence of and genes involved in UV resistance and DNA repair. These features indicate the spp adaptation to high UV radiation environments.
从中国西藏自治区采集的土壤样本中分离出四株革兰氏阳性、需氧、不运动且不形成芽孢的放线菌菌株（CPCC 206391、CPCC 206453、CPCC 206435和CPCC 206450）。这四株菌株的16S rRNA基因序列与[某科某属]的成员显示出密切关系，与[该属]有效命名物种的相似性为96.1 - 99.4%。在基于16S rRNA基因序列和核心基因组构建的系统发育树中，这些分离株聚集在[某科]谱系内的[某属]分支中。这些菌株与其系统发育邻株之间的基因组相关性指数值，包括平均核苷酸同一性（78.1 - 91.5%）和数字DNA - DNA杂交（19.8 - 41.0%），均低于物种界定阈值。这些基因组数据，结合它们的表型特征，支持它们在[某属]内的分类，代表四个新物种。因此，提出了[新物种1名称]（模式菌株CPCC 206391 = XZ253 = KCTC 59139）、[新物种2名称]（模式菌株CPCC 206453 = CXZ644 = KCTC 59274）、[新物种3名称]（模式菌株CPCC 206435 = CXZ904 = KCTC 59271）和[新物种4名称]（模式菌株CPCC 206450 = XZ100 = KCTC 59273）。这些菌株在高强度紫外线辐射下表现出稳定生长，这归因于存在参与紫外线抗性和DNA修复的[相关基因名称1]和[相关基因名称2]基因。这些特征表明[该属新物种]适应高紫外线辐射环境。
