从乳腺癌细胞中纯化一种癌胚抗原。
Purification of an onco-fetal antigen from mammary carcinoma cells.
作者信息
Czuppon A B
出版信息
J Cancer Res Clin Oncol. 1985;110(3):252-4. doi: 10.1007/BF00399285.
Abstract
Mammary carcinoma cell membranes were solubilized by a zwitterionic detergent, 3-(3-cholamidopropyl)-dimethyl-ammonio)-propanesulfonate (CHAPS). The solubilized membrane components were resolved by high pressure liquid chromatography (HPLC) using a styrogel protein column. One of the fractions was found to react specifically with anti-F9-antiserum in a solid-phase immunosorbent assay. Following recycling this fraction was purified to homogeneity and had an N-terminal amino acid residue of lysine. Its molecular weight was estimated to be 15,000 daltons. No comparable fraction could be detected in the solubilized membrane components of nonmalignant mammary tissue.
摘要
用两性离子去污剂3-(3-胆酰胺丙基)-二甲基-铵基)-丙烷磺酸盐(CHAPS)溶解乳腺癌细胞膜。使用苯乙烯凝胶蛋白柱通过高压液相色谱(HPLC)分离溶解的膜成分。在固相免疫吸附试验中发现其中一个组分与抗F9抗血清发生特异性反应。回收后，将该组分纯化至同质，其N端氨基酸残基为赖氨酸。估计其分子量为15,000道尔顿。在非恶性乳腺组织的溶解膜成分中未检测到类似的组分。
相似文献
1
Purification of an onco-fetal antigen from mammary carcinoma cells.从乳腺癌细胞中纯化一种癌胚抗原。
J Cancer Res Clin Oncol. 1985;110(3):252-4. doi: 10.1007/BF00399285.
2
Cost-effectiveness of using prognostic information to select women with breast cancer for adjuvant systemic therapy.利用预后信息为乳腺癌患者选择辅助性全身治疗的成本效益
Health Technol Assess. 2006 Sep;10(34):iii-iv, ix-xi, 1-204. doi: 10.3310/hta10340.
3
[Volume and health outcomes: evidence from systematic reviews and from evaluation of Italian hospital data].[容量与健康结果：来自系统评价和意大利医院数据评估的证据]
Epidemiol Prev. 2013 Mar-Jun;37(2-3 Suppl 2):1-100.
4
Tumour-associated antigens of mammary carcinomas recognized by human monoclonal antibody 1G12.人单克隆抗体1G12识别的乳腺癌肿瘤相关抗原
J Cancer Res Clin Oncol. 1991;117(4):367-76. doi: 10.1007/BF01630722.
5
Signs and symptoms to determine if a patient presenting in primary care or hospital outpatient settings has COVID-19.在基层医疗机构或医院门诊环境中，如果患者出现以下症状和体征，可判断其是否患有 COVID-19。
Cochrane Database Syst Rev. 2022 May 20;5(5):CD013665. doi: 10.1002/14651858.CD013665.pub3.
6
Home treatment for mental health problems: a systematic review.心理健康问题的居家治疗：一项系统综述
Health Technol Assess. 2001;5(15):1-139. doi: 10.3310/hta5150.
7
Sertindole for schizophrenia.用于治疗精神分裂症的舍吲哚。
Cochrane Database Syst Rev. 2005 Jul 20;2005(3):CD001715. doi: 10.1002/14651858.CD001715.pub2.
8
Inhaled mannitol for cystic fibrosis.吸入用甘露醇治疗囊性纤维化。
Cochrane Database Syst Rev. 2018 Feb 9;2(2):CD008649. doi: 10.1002/14651858.CD008649.pub3.
9
Strategies for detecting colon cancer and/or dysplasia in patients with inflammatory bowel disease.检测炎症性肠病患者结肠癌和/或发育异常的策略。
Cochrane Database Syst Rev. 2006 Apr 19(2):CD000279. doi: 10.1002/14651858.CD000279.pub3.
10
Educational interventions for the management of cancer-related fatigue in adults.针对成人癌症相关疲劳管理的教育干预措施。
Cochrane Database Syst Rev. 2016 Nov 24;11(11):CD008144. doi: 10.1002/14651858.CD008144.pub2.
本文引用的文献
1
Purification of a human spermatozoal antigen.一种人类精子抗原的纯化。
Hoppe Seylers Z Physiol Chem. 1981 Jul;362(7):963-8. doi: 10.1515/bchm2.1981.362.2.963.
2
Chemical synthesis of a decapeptide eliciting characteristics of a human spermatozoal antigen.一种引发人类精子抗原特性的十肽的化学合成。
Hoppe Seylers Z Physiol Chem. 1982 Dec;363(12):1465-71. doi: 10.1515/bchm2.1982.363.2.1465.
3
A nondenaturing zwitterionic detergent for membrane biochemistry: design and synthesis.一种用于膜生物化学的非变性两性离子去污剂：设计与合成。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6368-70. doi: 10.1073/pnas.77.11.6368.
4
Separation of porcine zona pellucida components by high-performance liquid chromatography on styro-gel protein columns.通过在Styro-gel蛋白质柱上进行高效液相色谱法分离猪透明带成分。
J Chromatogr. 1983 Jul 8;275(2):423-7. doi: 10.1016/s0378-4347(00)84390-x.
5
Solubilization of active opiate receptors.活性阿片受体的增溶作用。
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4623-7. doi: 10.1073/pnas.77.8.4623.
6
Analogies between embryonic (T/t) antigens and adult major histocompatibility (H-2) antigens.胚胎（T/t）抗原与成年主要组织相容性（H-2）抗原之间的类比。
Nature. 1975 Aug 14;256(5518):545-7. doi: 10.1038/256545a0.
7
[Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line (author's transl)].[小鼠畸胎癌：多能原始细胞系培养中的分化（作者译）]
Ann Microbiol (Paris). 1975 Jan;126(1):3-22.
8
Isolation of a human teratoma cell line which expresses F9 antigen.
Nature. 1977 Dec 8;270(5637):515-8. doi: 10.1038/270515a0.
9
On the occurrence of the pregnancy zone protein (PZ) in gynecological cancer.关于妇科癌症中妊娠区蛋白（PZ）的出现情况。
Arch Gynakol. 1976 Sep 17;221(2):97-101. doi: 10.1007/BF00667139.
10
Correlation of the blood-level of a pregnancy-associated alpha-macroglobulin with the clinical course of cancer patients.妊娠相关α-巨球蛋白血液水平与癌症患者临床病程的相关性。
Lancet. 1975 Apr 5;1(7910):777-9. doi: 10.1016/s0140-6736(75)92439-3.
Zotero 插件