Ma Zhixin, Wu Qi, Yu Hui, Pan Qiao, Liu Tong, Xin Jiuqing, Xu Qingyuan
State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Institute of Western Agriculture, Chinese Academy of Agricultural Sciences, Xinjiang, China.
J Bacteriol. 2025 Sep 18;207(9):e0016025. doi: 10.1128/jb.00160-25. Epub 2025 Aug 11.
is a highly infectious pathogenic microorganism that causes various clinical signs in cattle, including pneumonia, arthritis, and mastitis, often resulting in significant economic losses. The adhesion of to host cells is a pivotal step in the infection process, which is a complex process involving multiple pathogenic and host proteins. Molecules involved in adhesion and colonization are widely recognized as important virulence factors and often implicated in the infection and pathogenesis. In this study, the gene of was cloned, and the protein encoded by this gene was expressed and purified. The rabbit polyclonal antibody against this protein was also produced. This protein was shown to be a surface protein and to react with the -positive serum. The ability of this protein to adhere to host cells was verified using embryonic bovine lung (EBL) and Madin-Darby bovine kidney (MDBK) cells. Furthermore, the adhesion function of this protein was found to be achieved through interaction with heparin on the host cell surface, and the key region of the protein involved in heparin binding was identified. The conservation of the protein encoded by the gene was also analyzed. The results of this study suggest that the protein encoded by the gene is a heparin-binding surface membrane protein of associated with infection.IMPORTANCEMycoplasmas lack a cell wall, and the membrane proteins interacting with host cells play essential roles in their infection and proliferation processes. In this study, we identified a membrane protein encoded by that interacts with heparin on the surface of host cells. Heparin is widely distributed in various cells and tissues of the host and serves as a receptor for the infection and invasion of many pathogenic microorganisms. The ability of to invade multiple tissues may be related to its heparin-binding capacity. The heparin-binding protein identified in this study is valuable for further research on the infection and invasion mechanisms of .
是一种高度传染性的致病微生物，可导致牛出现各种临床症状，包括肺炎、关节炎和乳腺炎，常造成重大经济损失。其与宿主细胞的黏附是感染过程中的关键步骤，这是一个涉及多种致病蛋白和宿主蛋白的复杂过程。参与黏附和定植的分子被广泛认为是重要的毒力因子，常与感染和发病机制有关。在本研究中，克隆了其基因，并表达和纯化了该基因编码的蛋白质。还制备了针对该蛋白质的兔多克隆抗体。该蛋白质被证明是一种表面蛋白，并与阳性血清发生反应。使用胚胎牛肺（EBL）细胞和马迪达比牛肾（MDBK）细胞验证了该蛋白质黏附宿主细胞的能力。此外，发现该蛋白质的黏附功能是通过与宿主细胞表面的肝素相互作用实现的，并确定了该蛋白质中参与肝素结合的关键区域。还分析了该基因编码蛋白质的保守性。本研究结果表明，该基因编码的蛋白质是一种与感染相关的肝素结合表面膜蛋白。
重要性
支原体缺乏细胞壁，与宿主细胞相互作用的膜蛋白在其感染和增殖过程中起重要作用。在本研究中，我们鉴定了一种由编码的膜蛋白，它与宿主细胞表面的肝素相互作用。肝素广泛分布于宿主的各种细胞和组织中，是许多致病微生物感染和入侵的受体。其侵入多种组织的能力可能与其肝素结合能力有关。本研究中鉴定的肝素结合蛋白对于进一步研究其感染和入侵机制具有重要价值。
