Zhang Yiming, Song Minyan, He Yonghong, Si Yehua
School of Tourism Management, Huzhou Vocational and Technical College, Huzhou, China.
College of Forestry and Biotechnology, Zhejiang Agricultural and Forestry University, Hangzhou, China.
Front Microbiol. 2025 Jul 31;16:1612979. doi: 10.3389/fmicb.2025.1612979. eCollection 2025.
In recent years, root rot disease has occurred in Yunnan and caused significant economic losses to local plantation farmers, the pathogen was identified as through morphological and molecular method. The indoor toxicity testing and biological characteristic research on were carried out. Additionally, the transcriptional regulatory mechanism under different pH stress conditions was studied. The results showed that Prochloraz had the strongest inhibitory effect on mycelial growth, with an EC value of 0.059 mg·L-1. grows best in an environment of 26°C, 12: 12 alternating light, and pH 6. Go enrichment analysis revealed that the differentially expressed genes were mainly related to the biosynthesis of ribonucleoprotein complexes, ncRNA metabolic processes, ribosome and so on. KEGG analysis shows that in acidic environments, responds to external stress by precisely regulating amino acid metabolism and ribosome function, while in alkaline environments, it helps cells respond to and perceive external stress by forming multi-level adaptive networks and inhibiting activities such as protein synthesis transcription. This study provides relevant references for the prevention and control of this disease.
近年来，云南发生根腐病，给当地种植农户造成重大经济损失，通过形态学和分子学方法鉴定了病原菌。对该病原菌进行了室内毒力测定和生物学特性研究。此外，研究了不同pH胁迫条件下的转录调控机制。结果表明，咪鲜胺对该病原菌菌丝生长的抑制作用最强，EC值为0.059 mg·L-1。该病原菌在26℃、12:12光暗交替、pH 6的环境中生长最佳。基因本体富集分析表明，差异表达基因主要与核糖核蛋白复合体的生物合成、非编码RNA代谢过程、核糖体等有关。KEGG分析表明，在酸性环境中，该病原菌通过精确调节氨基酸代谢和核糖体功能来应对外部胁迫，而在碱性环境中，它通过形成多层次的适应性网络并抑制蛋白质合成转录等活动来帮助细胞应对和感知外部胁迫。本研究为该病的防治提供了相关参考。
