Aoki Hiroshi, Miyazaki Risa, Einaga Yasuaki
Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba 305-8569, Ibaraki, Japan.
Department of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Kanagawa, Japan.
Biosensors (Basel). 2025 Jul 29;15(8):489. doi: 10.3390/bios15080489.
Histamine sensing that uses enzymatic reactions is the most common form of testing due to its selectivity for histamine. However, enzymes are difficult to store for long periods of time, and the inactivation of enzymes decreases the reliability of the results. In this study, we developed a novel, quick, and easily operated histamine sensing technique that takes advantage of the histamine redox reaction and does not require enzyme-based processes. Because the redox potential of histamine is relatively high, we used a boron-doped diamond (BDD) electrode that has a wide potential window. At pH 8.4, which is between the acidity constant of histamine and the isoelectric point of histidine, it was found that an oxygen-terminated BDD surface successfully detected histamine, both selectively and exclusively. Measurements of the sensor's responses to extracts from fish meat samples that contained histamine at various concentrations revealed that the sensor responds linearly to the histamine concentration, thus allowing it to be used as a calibration curve. The sensor was used to measure histamine in another fish meat sample treated as an unknown sample, and the response was fitted to the calibration curve to perform an inverse estimation. When estimated in this way, the histamine concentration matched the certified value within the range of error. A more detailed examination showed that the sensor response was little affected by the histidine concentration in the sample. The detection limit was 20.9 ppm, and the linear response range was 0-150 ppm. This confirms that this sensing method can be used to measure standard histamine concentrations.
由于对组胺具有选择性，利用酶促反应的组胺传感是最常见的检测形式。然而，酶难以长时间储存，酶的失活会降低结果的可靠性。在本研究中，我们开发了一种新颖、快速且易于操作的组胺传感技术，该技术利用组胺氧化还原反应，无需基于酶的过程。由于组胺的氧化还原电位相对较高，我们使用了具有宽电位窗口的硼掺杂金刚石（BDD）电极。在组胺酸度常数和组氨酸等电点之间的pH 8.4条件下，发现氧终止的BDD表面能够成功地选择性且专一性地检测组胺。对含有不同浓度组胺的鱼肉样品提取物的传感器响应测量表明，该传感器对组胺浓度呈线性响应，因此可将其用作校准曲线。该传感器用于测量另一个作为未知样品处理的鱼肉样品中的组胺，并将响应拟合到校准曲线进行反推估计。以这种方式估计时，组胺浓度在误差范围内与认证值匹配。更详细的检查表明，传感器响应受样品中组氨酸浓度的影响很小。检测限为20.9 ppm，线性响应范围为0 - 150 ppm。这证实了这种传感方法可用于测量标准组胺浓度。
