da Costa Fábio Burack, Cavalcanti Andrea Cony, Erbisti Rafael Santos, Dias Vanessa Zaquieu, Moreira Cristiane Gomes de Castro, Grifo Mateus Marques, Vaz Maria Carmelita Dos Santos, Camargo Adriana Cardoso, de Souza Leandro Magalhães, Dos Santos Flávia Barreto, Ribeiro Mário Sérgio, Malirat Viviana, Alves Honório Nildimar, Azevedo Renata Campos
Laboratório das Interações Vírus-Hospedeiros-LIVH, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro CEP 21040-360, RJ, Brazil.
Departamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-902, RJ, Brazil.
Pathogens. 2025 Aug 21;14(8):833. doi: 10.3390/pathogens14080833.
The Oropouche virus (OROV) has been circulating in the Amazon region since the 1960s, with a progressive increase in outbreaks and human cases reported in Brazil and neighboring countries. In the Rio de Janeiro state, there has been a significant rise in suspected cases of arboviruses, with only 30% of laboratory tests confirming infections with dengue, Zika, or chikungunya viruses. The investigation of OROV virus circulation in the Rio de Janeiro state was initiated and confirmed in April 2024. Our study aimed to retrospectively investigate OROV infections in dengue-suspected cases with inconclusive diagnosis in order to better understand the temporal and geographic introduction of OROV in the Rio de Janeiro state. Municipalities from Rio de Janeiro with arbovirus-like fever cases but a low percentage of dengue-positive RT-PCR test confirmations were identified in the laboratory database. Samples were selected for testing OROV infections using real-time RT-PCR as recommended by the Brazilian Ministry of Health. Municipalities in the Middle Paraíba region of the state showed 93% negative tests results for dengue, Zika, and chikungunya starting in September 2023. A total of 118 positive cases of Oropouche were recorded in the state of Rio de Janeiro between March and July 2024. Moreover, by genome sequencing of eight strains, it was shown that OROV circulating in Rio de Janeiro belongs to recently emergent MLS lineage. Our findings retrospectively revealed a concentration of cases in the Middle Paraíba region and an outbreak in the rural village of Cacaria, located in the municipality of Piraí. According to our data, this region is the first area with sustained transmission in the Rio de Janeiro state.
奥罗普切病毒（OROV）自20世纪60年代以来一直在亚马逊地区传播，巴西及周边国家报告的疫情和人类病例呈逐渐上升趋势。在里约热内卢州，虫媒病毒疑似病例显著增加，只有30%的实验室检测确认感染了登革热、寨卡或基孔肯雅病毒。2024年4月启动并确认了对里约热内卢州OROV病毒传播情况的调查。我们的研究旨在回顾性调查诊断不明确的登革热疑似病例中的OROV感染情况，以便更好地了解OROV在里约热内卢州的时间和地理引入情况。在实验室数据库中确定了里约热内卢出现虫媒病毒样发热病例但登革热阳性RT-PCR检测确认率较低的市。按照巴西卫生部的建议，选择样本使用实时RT-PCR检测OROV感染情况。该州中帕拉伊巴地区的市自2023年9月起登革热、寨卡和基孔肯雅病毒检测结果93%为阴性。2024年3月至7月期间，里约热内卢州共记录了118例奥罗普切病毒阳性病例。此外，通过对8个毒株的基因组测序表明，在里约热内卢传播的OROV属于最近出现的MLS谱系。我们的研究结果回顾性地揭示了中帕拉伊巴地区病例集中以及位于皮拉伊市的卡卡里亚乡村爆发疫情。根据我们的数据，该地区是里约热内卢州首个出现持续传播的地区。
