Wang Lidan, Sun Weisheng, Jiang Wenhao, Yang Zhuanying, Lin Dongbo
College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, Guangdong, PR China.
South Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, 524013, Guangdong, PR China.
BMC Plant Biol. 2025 Sep 4;25(1):1196. doi: 10.1186/s12870-025-07292-1.
The Aux/IAA protein is integral to the modulation of auxin signaling, which is essential for plant growth and development. However, systematic analysis on the Aux/IAA gene family in pineapple ( L.) remains unexplored.
We systematically identified 20 Aux/IAA genes in pineapple, classifying them into eight evolutionary groups. Structural analysis revealed conserved domains (I-IV) and intron-exon patterns, with most genes harboring hormone/stress-responsive -elements. Subcellular localization confirmed nuclear targeting of all AcIAA-GFP fusion proteins. Tissue-specific expression profiling identified as root-enriched, as floral-specific, and as leaf-preferential. During fruit development, peaked at ripening stage, while declined progressively. Yeast two-hybrid assays uncovered 68 AcIAA-AcARF interactions, with AcARF6/14 showing broad binding capacity, while AcIAA1/17/20 exhibited no interactions. These findings present insights into pineapple genes, revealing their structural diversity, spatiotemporal expression dynamics, and interaction networks with AcARFs, and highlight their potential roles in organ development and fruit ripening.
This study establishes a foundation for future functional analyses aimed at elucidating the molecular mechanisms underlying auxin signaling, which governs fruit yield and quality in pineapples.
The online version contains supplementary material available at 10.1186/s12870-025-07292-1.
Aux/IAA蛋白对于生长素信号转导的调节至关重要，而生长素信号转导对植物生长发育必不可少。然而，对菠萝（Ananas comosus (L.)）中Aux/IAA基因家族的系统分析仍未开展。
我们在菠萝中系统鉴定出20个Aux/IAA基因，将它们分为八个进化组。结构分析揭示了保守结构域（I-IV）和内含子-外显子模式，大多数基因含有激素/胁迫响应元件。亚细胞定位证实所有AcIAA-GFP融合蛋白均定位于细胞核。组织特异性表达谱分析确定某些基因在根中富集，某些基因具有花特异性，某些基因优先在叶中表达。在果实发育过程中，某些基因在成熟阶段达到峰值，而某些基因则逐渐下降。酵母双杂交试验揭示了68对AcIAA-AcARF相互作用，AcARF6/14显示出广泛的结合能力，而AcIAA1/17/20没有相互作用。这些发现为菠萝Aux/IAA基因提供了见解，揭示了它们的结构多样性、时空表达动态以及与AcARF的相互作用网络，并突出了它们在器官发育和果实成熟中的潜在作用。
本研究为未来旨在阐明生长素信号转导分子机制的功能分析奠定了基础，生长素信号转导控制着菠萝的果实产量和品质。
在线版本包含可在10.1186/s12870-025-07292-1获取的补充材料。
