Yurkov Andrey P, Puzanskiy Roman K, Kryukov Alexey A, Kudriashova Tatyana R, Kovalchuk Anastasia I, Gorenkova Anastasia I, Bogdanova Ekaterina M, Laktionov Yuri V, Romanyuk Daria A, Yemelyanov Vladislav V, Shavarda Alexey L, Shishova Maria F
Laboratory of Ecology of Symbiotic and Associative Rhizobacteria, All-Russia Research Institute for Agricultural Microbiology, Pushkin, St. Petersburg 196608, Russia.
Laboratory of Analytical Phytochemistry, Komarov Botanical Institute of the Russian Academy of Sciences, St. Petersburg 197022, Russia.
Plants (Basel). 2025 Aug 28;14(17):2685. doi: 10.3390/plants14172685.
The arbuscular mycorrhizal fungi (AMF) effect on the plant metabolome is an actual question of plant biology. Its alteration during host plant development and at different phosphorus supplies is of special interest. The aim of this study was to evaluate the effect of (Błaszk., Wubet, Renker & Buscot) C. Walker & A. Schüßler inoculation and/or phosphorus treatment on the root metabolome of L. subsp. Koch at the first true leaf, second leaf, third leaf development stages, the lateral branching initiation, the flowering and the mature fruit stages. The assessment of metabolic profiles was performed using GC-MS. In total, 327 metabolites were annotated: among them 20 carboxylic acids, 26 amino acids, 14 fatty acids and 58 sugars. The efficient AM was characterized by the upregulation of the metabolism of proteins, carbohydrates and lipids, as well as an increase in the content of phosphates. The tricarboxylic acid abundance was generally lower during mycorrhization. Fourteen metabolic markers of the efficient AM symbiosis were identified. The lateral branching initiation stage was shown to have key importance. Long-lasting metabolomic profiling indicated variances in mycorrhization and Pi supply effects at different key stages of host plant development.
丛枝菌根真菌（AMF）对植物代谢组的影响是植物生物学领域的一个现实问题。其在宿主植物发育过程中以及不同磷供应条件下的变化尤为引人关注。本研究的目的是评估接种（Błaszk.、Wubet、Renker & Buscot）C. Walker & A. Schüßler和/或进行磷处理对亚种L. subsp. Koch在第一片真叶、第二片叶、第三片叶发育阶段、侧枝起始、开花和成熟果实阶段的根系代谢组的影响。使用气相色谱 - 质谱联用仪（GC-MS）对代谢谱进行评估。总共注释了327种代谢物：其中包括20种羧酸、26种氨基酸、14种脂肪酸和58种糖类。高效的丛枝菌根表现为蛋白质、碳水化合物和脂质代谢上调，以及磷酸盐含量增加。菌根形成过程中三羧酸的丰度通常较低。确定了14种高效丛枝菌根共生的代谢标记。结果表明侧枝起始阶段至关重要。长期的代谢组学分析表明，在宿主植物发育的不同关键阶段，菌根形成和磷供应效应存在差异。
