Sun Qisen, Song Zhubin, Li Fanghao, Zhu Xinyu, Zhang Xinyu, Chen Hao
SDU-ANU Joint Science College, Shandong University, Weihai 264209, China.
Marine College, Shandong University, Weihai 264209, China.
Molecules. 2025 Aug 23;30(17):3469. doi: 10.3390/molecules30173469.
An innovative ultrasound-assisted deep eutectic solvent-based three-phase partitioning (UA-DES-TPP) system was developed for the sustainable extraction of polysaccharide (FCP). Using a hydrophobic DES composed of dodecanoic acid and octanoic acid (1:1 molar ratio), a phase behavior-driven separation mechanism was established. The system was systematically optimized through single-factor experiments and response surface methodology (RSM), achieving a maximum FCP yield of 9.22 ± 0.20% under optimal conditions (liquid-solid ratio 1:24.2 g/mL, top/bottom phase volume ratio 1:1.05 /, ammonium sulfate concentration 25.8%). Structural characterization revealed that FCP was a heteropolysaccharide primarily composed of glucose and mannose with α/β-glycosidic linkages and a loose fibrous network. Remarkably, the DESs demonstrated excellent recyclability over five cycles. Furthermore, FCP exhibited significant concentration-dependent antioxidant activities: 82.3 ± 3.8% DPPH radical scavenging at 8 mg/mL, 76.8 ± 0.8% ABTS scavenging, and ferric ion reducing power of 45.53 ± 1.07 μmol TE/g. This study provides a new path for the efficient and sustainable extraction of bioactive macromolecules.
开发了一种创新的超声辅助深共晶溶剂基三相分配（UA-DES-TPP）系统，用于可持续提取多糖（FCP）。使用由十二烷酸和辛酸（摩尔比1:1）组成的疏水性深共晶溶剂，建立了相行为驱动的分离机制。通过单因素实验和响应面法（RSM）对该系统进行了系统优化，在最佳条件下（液固比1:24.2 g/mL、上下相体积比1:1.05、硫酸铵浓度25.8%），FCP的最大产率达到9.22±0.20%。结构表征表明，FCP是一种杂多糖，主要由葡萄糖和甘露糖组成，具有α/β-糖苷键和疏松的纤维网络。值得注意的是，深共晶溶剂在五个循环中表现出优异的可回收性。此外，FCP表现出显著的浓度依赖性抗氧化活性：在8 mg/mL时，DPPH自由基清除率为82.3±3.8%，ABTS清除率为76.8±0.8%，铁离子还原能力为45.53±1.07 μmol TE/g。本研究为生物活性大分子的高效可持续提取提供了一条新途径。
