Stanley Merita, Rajula Maria Antony Prem Blaise, Shankar Potluri Leela Ravi, Rao Karkala Sunanda, Sairam Amrutha, Jeyakumaran Sanjana
Department of Periodontology, SRM Kattankulathur Dental College and Hospital, SRM Institute of Science and Technology, Chengalpattu, Tamil Nadu, India.
J Indian Soc Periodontol. 2025 Mar-Apr;29(2):147-152. doi: 10.4103/jisp.jisp_461_24. Epub 2025 Aug 19.
Periodontal disease is a chronic inflammatory condition that leads to the destruction of periodontal tissues, exacerbated by bacterial lipopolysaccharide (LPS). Current therapeutic approaches seek to reduce inflammation and promote regeneration. Curcumin, a natural polyphenol with anti-inflammatory and antioxidant properties, may offer a biocompatible alternative for periodontal regeneration by protecting human periodontal ligament cells (hPDLCs) from inflammatory damage.
The present study examined the effects of curcumin on hPDLCs exposed to LPS (10 µg/mL) to simulate an inflammatory environment. hPDLCs were divided into four groups: control, curcumin-treated (2.5 µM and 5 µM), LPS-induced inflammation, and LPS with curcumin. Cell viability was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and morphological changes were evaluated via phase-contrast microscopy.
Curcumin treatment did not significantly impact cell viability in hPDLCs under noninflammatory conditions, with viability rates exceeding 94%. In LPS-stimulated cells, curcumin at 2.5 µM and 5 µM significantly improved viability from 60.1% (LPS only) to 80.1% and 87.6%, respectively. Morphological assessment revealed that curcumin mitigated LPS-induced cellular damage, promoting normal cell structure and growth.
Curcumin demonstrates potential as a therapeutic agent for periodontal regeneration by enhancing cell viability and counteracting LPS-induced cytotoxicity in hPDLCs. This study supports curcumin's role in creating a microenvironment conducive to periodontal tissue repair and highlights its promise for future applications in periodontal therapy.
牙周病是一种慢性炎症性疾病，会导致牙周组织破坏，细菌脂多糖（LPS）会加剧这种破坏。目前的治疗方法旨在减轻炎症并促进组织再生。姜黄素是一种具有抗炎和抗氧化特性的天然多酚，通过保护人牙周膜细胞（hPDLCs）免受炎症损伤，可能为牙周再生提供一种生物相容性替代方案。
本研究检测了姜黄素对暴露于LPS（10μg/mL）以模拟炎症环境的hPDLCs的影响。hPDLCs分为四组：对照组、姜黄素处理组（2.5μM和5μM）、LPS诱导炎症组以及LPS与姜黄素共同处理组。使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法评估细胞活力，并通过相差显微镜评估形态变化。
在非炎症条件下，姜黄素处理对hPDLCs的细胞活力没有显著影响，存活率超过94%。在LPS刺激的细胞中，2.5μM和5μM的姜黄素显著提高了细胞活力，分别从仅LPS处理时的60.1%提高到80.1%和87.6%。形态学评估显示，姜黄素减轻了LPS诱导的细胞损伤，促进了正常的细胞结构和生长。
姜黄素通过提高细胞活力和抵消LPS诱导的hPDLCs细胞毒性，显示出作为牙周再生治疗剂的潜力。本研究支持姜黄素在创造有利于牙周组织修复的微环境中的作用，并突出了其在未来牙周治疗中的应用前景。
