独特型抗体的研究。自身抗独特型抗血清的产生与特性鉴定。
Studies of idiotypic antibodies. Production and characterization of autoantiidiotypic antisera.
作者信息
Rodkey L S
出版信息
J Exp Med. 1974 Mar 1;139(3):712-20. doi: 10.1084/jem.139.3.712.
Rabbits were immunized with a hapten-protein conjugate and sera were collected for 189 days. The antihapten antibodies were purified by affinity chromatography, then the same animal that synthesized the antibody was reinjected with polymerized F(ab')(2) fragments of antihapten antibodies. Sera were collected after autoimmunization and tested by an indirect radioimmunoassay technique for reaction with [(125)I]F(ab')(2) fragments of the original antihapten antibody. Results showed that each individual responded to its own F(ab')(2) and the antisera were specific for antihapten antibodies of that individual. Quantitative allotype assays established the immunoglobulin nature of the labeled test antigen. Inhibition assays showed that the reaction was specifically inhibitable with hapten. The relationship of this system with other idiotypic systems and the possible autoimmune implications of autoantiidiotypic antibodies are discussed.
用半抗原 - 蛋白质偶联物免疫兔子，并收集血清189天。通过亲和层析纯化抗半抗原抗体，然后将合成该抗体的同一只动物再次注射抗半抗原抗体的聚合F(ab')(2)片段。自身免疫后收集血清，并通过间接放射免疫测定技术检测其与原始抗半抗原抗体的[(125)I]F(ab')(2)片段的反应。结果显示，每个个体对自身的F(ab')(2)有反应，并且抗血清对该个体的抗半抗原抗体具有特异性。定量同种异型测定确定了标记测试抗原的免疫球蛋白性质。抑制试验表明，该反应可被半抗原特异性抑制。讨论了该系统与其他独特型系统的关系以及自身抗独特型抗体可能的自身免疫影响。
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