Bai Xi, Ma Liangru, Li Anjian, Guo Taozong, Hu Hong, Pan Hongbin
Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China; College of Animal Science, Anhui Science and Technology University, Bengbu, 233000, China.
Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.
Poult Sci. 2025 Sep 18;104(11):105850. doi: 10.1016/j.psj.2025.105850.
The present work investigated the effects of pantethine on egg quality, lipid parameters, lipid metabolism-related enzymes, hepatic gene expression, and gut microbiota in laying hens. A total of 144 46-week-old laying hens were randomly assigned to two treatments (6 replicates × 12 birds per group): a basic diet without pantethine (CK) and a basic diet supplemented with 200 mg/kg pantethine (TP). Dietary pantethine maintained productive performance and increased egg Haugh unit (P < 0.05). Serum and hepatic triglyceride contents were reduced in pantethine-treated laying hens (P < 0.05). Dietary pantethine elevated lipoprotein lipase and total lipase levels, while decreasing fatty acid synthase and acetyl-CoA carboxylase levels in the liver (P < 0.05). Principal coordinate analysis revealed that dietary pantethine altered the hepatic gene profiles (P < 0.05). Liver transcriptomic analysis identified 427 genes showing significant expression changes in hepatic tissues of the TP group compared with CK group, including 276 upregulated and 151 downregulated genes. KEGG analysis showed enrichment of these genes in the PPAR signaling pathway, involving PPARG, PCK1, PLIN1, and SCD. For intestinal microbiota , dietary pantethine significantly changed cecal microbiota structure. Firmicutes and Bacteroidetes were the dominant phyla, but pantethine significantly reduced Firmicutes abundance and the Firmicutes/Bacteroidetes ratio in the cecal microbiota (P < 0.05). Burkholderia, Veillonella, and Mycobacterium were positively associated with PPARG but negatively related to PCK1, SCD, and PLIN1. In conclusion, hepatic genes and gut bacteria interacted synergistically to improve egg Haugh unit and hepatic lipid metabolism in pantethine-treated laying hens.
本研究探讨了泛硫乙胺对蛋鸡产蛋品质、脂质参数、脂质代谢相关酶、肝脏基因表达及肠道微生物群的影响。选取144只46周龄蛋鸡，随机分为两组（每组6个重复，每个重复12只鸡）：一组饲喂不含泛硫乙胺的基础日粮（CK），另一组饲喂添加200 mg/kg泛硫乙胺的基础日粮（TP）。日粮泛硫乙胺维持了蛋鸡的生产性能，并提高了蛋的哈氏单位（P < 0.05）。泛硫乙胺处理的蛋鸡血清和肝脏甘油三酯含量降低（P < 0.05）。日粮泛硫乙胺提高了脂蛋白脂肪酶和总脂肪酶水平，同时降低了肝脏中脂肪酸合成酶和乙酰辅酶A羧化酶水平（P < 0.05）。主坐标分析表明，日粮泛硫乙胺改变了肝脏基因图谱（P < 0.05）。肝脏转录组分析发现，与CK组相比，TP组肝脏组织中有427个基因表达发生显著变化，其中276个基因上调，151个基因下调。KEGG分析表明，这些基因在PPAR信号通路中富集，涉及PPARG、PCK1、PLIN1和SCD。对于肠道微生物群，日粮泛硫乙胺显著改变了盲肠微生物群结构。厚壁菌门和拟杆菌门是主要菌门，但泛硫乙胺显著降低了盲肠微生物群中厚壁菌门的丰度和厚壁菌门/拟杆菌门的比例（P < 0.05）。伯克霍尔德菌、韦荣球菌和分枝杆菌与PPARG呈正相关，但与PCK1、SCD和PLIN1呈负相关。总之，肝脏基因和肠道细菌协同作用，改善了泛硫乙胺处理蛋鸡的蛋哈氏单位和肝脏脂质代谢。
