Yu Huizhi, Lei Youyan, Ma Ling, He Xiahong, Dai Wenhao, Chen Jie, Hao Xin
Yunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Southwest Forestry University, Kunming 650224, China.
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China.
J Fungi (Basel). 2025 Aug 22;11(9):613. doi: 10.3390/jof11090613.
, a tropical and subtropical fruit tree with significant economic and nutritional value, faces serious fungal disease problems during cultivation that severely affect yield and quality. In November 2024, leaf blight symptoms of were observed in Menglian, Pu'er, Yunnan, China, with a disease incidence of 23% in the field. Initial symptoms included small spots that enlarged into circular to irregular lesions with red-brown centers and brown to black margins. Finally, the leaves turned yellow and became scorched, eventually leading to massive leaf shedding. Infected leaf samples were collected, and fungal strains were isolated, purified, and inoculated via spore suspension, followed by re-isolation. The strains were conclusively identified as (SWFUCB2, SWFUCB1) through an integrated approach combining DNA extraction, polymerase chain reaction (PCR), sequencing, phylogenetic reconstruction, and morphological characterization. This is the first report of causing leaf blight disease in China, filling a gap in research on this disease. This study provided important information for epidemiological research on this disease and the development of comprehensive leaf blight disease control strategies.
作为一种具有重要经济和营养价值的热带及亚热带果树，在栽培过程中面临严重的真菌病害问题，这严重影响了产量和品质。2024年11月，在中国云南普洱孟连观察到该果树的叶枯病症状，田间发病率为23%。最初症状为小斑点，后扩大成圆形至不规则病斑，病斑中心为红棕色，边缘为棕色至黑色。最后，叶片变黄并焦枯，最终导致大量落叶。采集了受感染的叶片样本，分离、纯化真菌菌株，并通过孢子悬浮液接种，随后进行再分离。通过结合DNA提取、聚合酶链反应（PCR）、测序、系统发育重建和形态学特征的综合方法，最终确定这些菌株为（SWFUCB2、SWFUCB1）。这是在中国首次报道该病菌引起叶枯病，填补了该病研究的空白。本研究为该病的流行病学研究及叶枯病综合防治策略的制定提供了重要信息。
