Yu Huizhi, Lei Youyan, Ma Ling, He Xiahong, Dai Wenhao, Chen Jie, Hao Xin
Yunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource, Southwest Forestry University, Kunming 650224, China.
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China.
J Fungi (Basel). 2025 Aug 22;11(9):613. doi: 10.3390/jof11090613.
, a tropical and subtropical fruit tree with significant economic and nutritional value, faces serious fungal disease problems during cultivation that severely affect yield and quality. In November 2024, leaf blight symptoms of were observed in Menglian, Pu'er, Yunnan, China, with a disease incidence of 23% in the field. Initial symptoms included small spots that enlarged into circular to irregular lesions with red-brown centers and brown to black margins. Finally, the leaves turned yellow and became scorched, eventually leading to massive leaf shedding. Infected leaf samples were collected, and fungal strains were isolated, purified, and inoculated via spore suspension, followed by re-isolation. The strains were conclusively identified as (SWFUCB2, SWFUCB1) through an integrated approach combining DNA extraction, polymerase chain reaction (PCR), sequencing, phylogenetic reconstruction, and morphological characterization. This is the first report of causing leaf blight disease in China, filling a gap in research on this disease. This study provided important information for epidemiological research on this disease and the development of comprehensive leaf blight disease control strategies.