叶口蝠科蝙蝠（翼手目：叶口蝠科）中的重复DNA与核型进化

Repetitive DNAs and Karyotype Evolution in Phyllostomid Bats (Chiroptera: Phyllostomidae).

作者信息

Deon Geize Aparecida, Ezaz Tariq, Stornioli José Henrique Forte, Dos Santos Rodrigo Zeni, Gomes Anderson José Baia, Setti Príncia Grejo, de Oliveira Edivaldo Herculano Correa, Porto-Foresti Fábio, Utsunomia Ricardo, Liehr Thomas, Cioffi Marcelo de Bello

机构信息

Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos 13565-905, São Paulo, Brazil.

Institute of Applied Ecology, Faculty of Science and Technology, University of Canberra, Australian Capital Territory, Canberra 2617, Australia.

出版信息

Biomolecules. 2025 Aug 29;15(9):1248. doi: 10.3390/biom15091248.

DOI:10.3390/biom15091248

PMID:41008555

Abstract

Bats are great models for studying repetitive DNAs due to their compact genomes and extensive chromosomal rearrangements. Here, we investigated the repetitive DNA content of two phyllostomid bat species, (2 = 30♀/31♂) and (2 = 20♀/21♂), both harboring a multiple XYY sex chromosome system. Satellite DNA (satDNA) libraries were isolated and characterized, revealing four and ten satDNA families in and , respectively. These sequences, along with selected microsatellites, were in situ mapped onto chromosomes in both species and phylogenetically related taxa. SatDNAs showed strong accumulation in centromeric and subtelomeric regions, especially pericentromeric areas. Cross-species mapping with -derived probes indicated terminal localization patterns in other bat species, suggesting conserved distribution. Microsatellites co-localized with 45S rDNA clusters on the neo-sex chromosomes. Additionally, genomic hybridization revealed a male-specific signal on the Y chromosome, pointing to potential sex-linked repetitive regions. These findings confirm that bat genomes display relatively low amounts of repetitive DNA compared to other mammals and underscore the role of these elements in genome organization and sex chromosome evolution in phyllostomid bats.

摘要

由于蝙蝠基因组紧凑且染色体重排广泛，它们是研究重复DNA的优秀模型。在此，我们研究了两种叶口蝠科蝙蝠物种（Artibeus lituratus，2n = 30♀/31♂和Carollia perspicillata，2n = 20♀/21♂）的重复DNA含量，这两种蝙蝠都拥有多重XYY性染色体系统。分离并鉴定了卫星DNA（satDNA）文库，在Artibeus lituratus和Carollia perspicillata中分别发现了4个和10个satDNA家族。这些序列以及选定的微卫星被原位定位到这两个物种以及系统发育相关类群的染色体上。SatDNA在着丝粒和亚端粒区域，尤其是着丝粒周围区域有强烈积累。用Artibeus lituratus衍生的探针进行跨物种定位表明在其他蝙蝠物种中存在末端定位模式，提示分布保守。微卫星与新性染色体上的45S rDNA簇共定位。此外，基因组杂交在Y染色体上显示出雄性特异性信号，表明存在潜在的性连锁重复区域。这些发现证实，与其他哺乳动物相比蝙蝠基因组的重复DNA含量相对较低，并强调了这些元件在叶口蝠科蝙蝠基因组组织和性染色体进化中的作用。