Baquedano María Soledad, Stoore Caroll, Hidalgo Christian, Pereira Ismael, Paredes Rodolfo
Laboratorio Medicina Veterinaria, Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370035, Chile.
Núcleo de Investigación en One Health (NIOH), Facultad de Medicina Veterinaria y Agronomía, Universidad de las Américas, Santiago Centro 8370065, Chile.
Int J Mol Sci. 2025 Sep 22;26(18):9236. doi: 10.3390/ijms26189236.
Cystic echinococcosis (CE) is a globally distributed zoonotic disease caused by sensu lato, forming fluid-filled cysts in humans and livestock. These cysts consist of three layers: an inner germinal layer and a middle laminar layer of parasitic origin, and an outer adventitial layer derived from the host's immune response. The adventitial layer typically contains immune cells such as T and B lymphocytes, macrophages, and other inflammatory cells. Notably, differences have been reported in the cellular composition of this layer depending on the host species. However, the variation in calprotectin expression-a protein specific to phagocytes-between cattle and sheep CE cysts has not been previously described. This study assessed calprotectin expression using immunohistochemistry with anti-calprotectin antibodies on adventitial tissue sections from cattle and sheep CE cysts. The results showed a significantly higher calprotectin expression in the adventitial layer of cattle cysts compared to sheep. This difference was not associated with the fertility or anatomical location of the cysts. These findings suggest that the host species influences the level of calprotectin expression in the adventitial layer, contributing to our understanding of host-specific immune responses in CE.
囊型包虫病（CE）是一种由细粒棘球绦虫引起的全球性分布的人畜共患病，可在人和家畜体内形成充满液体的囊肿。这些囊肿由三层组成：内层生发层和中间源于寄生虫的板层层，以及外层外膜层，其源自宿主的免疫反应。外膜层通常含有免疫细胞，如T和B淋巴细胞、巨噬细胞及其他炎症细胞。值得注意的是，根据宿主物种不同，该层的细胞组成存在差异。然而，此前尚未描述牛和羊CE囊肿之间吞噬细胞特异性蛋白钙卫蛋白表达的差异。本研究使用抗钙卫蛋白抗体通过免疫组织化学方法评估了牛和羊CE囊肿外膜组织切片中钙卫蛋白的表达。结果显示，与羊相比，牛囊肿外膜层中钙卫蛋白的表达显著更高。这种差异与囊肿的育性或解剖位置无关。这些发现表明宿主物种会影响外膜层中钙卫蛋白的表达水平，有助于我们理解CE中宿主特异性免疫反应。
