Zhao Zirun, Liu Rou, Yu Jiawen, Liu Yunlong, Li Mingchun, Yu Qilin
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, College of Life Sciences, Nankai University, Tianjin 300071, China.
Molecules. 2025 Sep 9;30(18):3669. doi: 10.3390/molecules30183669.
Soil saline-alkali stress is a major problem faced by global agriculture, and there is an urgent need to develop efficient amelioration strategies. While both probiotics and plant stress-resistant molecules play critical roles in the alleviation of crop stress, their efficient retention in crop rhizosphere regions remains a great challenge. In this study, the nanocarrier ZIF-8@SPBP@betaine (ZSBet) was constructed by introduction of the synthesized polysaccharide-binding protein (SPBP) and the stress-resistant molecule betaine to the metal-organic framework ZIF-8. During co-incubation, the probiotic and ZSBet efficiently bound together to form ZSBet + Novo co-assemblies, i.e., the integrated protein-ZIF-8-probiotic complexes mediated by polysaccharide-receptor recognition, which exhibited strong root-binding abilities. Microbiome analysis revealed that ZSBet + Novo reduced the α-diversity of rhizosphere bacteria and increased the absolute abundance of biofilm formation-related bacteria, e.g., , , and . During wheat cultivation in saline-alkali soil, ZSBet + Novo reduced soil pH by 0.63 units, decreased soil salt content by 0.11 g/kg, and increased soil nutrient levels. Furthermore, the co-assembly enhanced the wheat grain number by 145.05% and reduced root malondialdehyde and proline contents by 42.00% and 39.13%, respectively. This study provides a new strategy for improving crop resistance under saline-alkali stress in combination with nanotechnology and synthetic biology.
土壤盐碱胁迫是全球农业面临的一个主要问题，迫切需要制定有效的改良策略。虽然益生菌和植物抗逆分子在缓解作物胁迫方面都发挥着关键作用，但它们在作物根际区域的有效留存仍然是一个巨大的挑战。在本研究中，通过将合成的多糖结合蛋白（SPBP）和抗逆分子甜菜碱引入金属有机框架ZIF-8构建了纳米载体ZIF-8@SPBP@甜菜碱（ZSBet）。在共培养过程中，益生菌与ZSBet有效结合在一起形成ZSBet + Novo共组装体，即由多糖-受体识别介导的整合蛋白-ZIF-8-益生菌复合物，其表现出很强的根结合能力。微生物群落分析表明，ZSBet + Novo降低了根际细菌的α多样性，并增加了与生物膜形成相关细菌的绝对丰度，例如， ， ，以及 。在盐碱地种植小麦期间，ZSBet + Novo使土壤pH值降低了0.63个单位，土壤盐分含量降低了0.11 g/kg，并提高了土壤养分水平。此外，该共组装体使小麦粒数增加了145.05%，并使根中丙二醛和脯氨酸含量分别降低了42.00%和39.13%。本研究结合纳米技术和合成生物学提供了一种提高作物在盐碱胁迫下抗性的新策略。
