Li Tao, Liao Binfan, Li Danfeng, Zhang Jie, Zhao Chunhui, Pei Yunfei, Chen Liping, Wang Meng, Liu Yawen, Wu Xi, Huang Weijin, Nie Jianhui
Division of HIV/AIDS and Sexually-Transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control (NIFDC), Beijing 102629, China.
State Key Laboratory of Drug Regulatory Science, Beijing 102629, China.
Pathogens. 2025 Sep 10;14(9):910. doi: 10.3390/pathogens14090910.
Establish an in vivo evaluation model focused on the attachment and entry stages of Hendra virus infection for protective immunity assessment.
A golden hamster infection model based on recombinant Hendra-F/G pseudovirus was developed, and a luciferase luminescence assay was used to assess the optimal pseudoviral challenge in terms of route of infection, dose and detection time. The biodistribution of the pseudovirus in infected organs was evaluated using the IVIS spectral CT system. The protective effect of antibody prophylaxis was evaluated by measuring the luminescence intensity of pseudoviruses.
Intraperitoneal injection was identified as the optimal route of infection, and the optimal time of detection was 6 h post-challenge. Our model simulates the infection of the brain and lungs by live viruses, with the strongest infection occurring in the abdomen, especially in the intestinal organs. The dose of pseudovirus was linearly correlated with luminescence intensity. The infection model was able to differentiate the protective effect of monoclonal antibodies, with complete protection in the high-dose group.
The recombinant Hendra-F/G pseudovirus hamster model allows the effective evaluation of prophylactic monoclonal antibodies, providing a crucial tool for studying Hendra virus infection and control strategies.
建立一种聚焦于亨德拉病毒感染的附着和进入阶段的体内评估模型，用于保护性免疫评估。
构建了基于重组亨德拉 - F/G假病毒的金黄仓鼠感染模型，并使用荧光素酶发光测定法从感染途径、剂量和检测时间方面评估最佳假病毒攻击条件。使用IVIS光谱CT系统评估假病毒在受感染器官中的生物分布。通过测量假病毒的发光强度评估抗体预防的保护效果。
确定腹腔注射为最佳感染途径，最佳检测时间为攻击后6小时。我们的模型模拟了活病毒对脑和肺的感染，腹部尤其是肠道器官的感染最强。假病毒剂量与发光强度呈线性相关。该感染模型能够区分单克隆抗体的保护效果，高剂量组有完全保护作用。
重组亨德拉 - F/G假病毒仓鼠模型能够有效评估预防性单克隆抗体，为研究亨德拉病毒感染及控制策略提供了关键工具。
